Our journey begins with the profound issue of microplastic pollution in coastal waters. The prevalence of microplastics, particularly those originating from the degradation of Polyethylene terephthalate (PET), has sparked growing concern. These minute plastic particles have infiltrated marine environments, posing a significant threat to ecosystems and human health.

Microbes have emerged as critical players in pollutant remediation and material cycling within ecosystems. Since the 1990s, microbial enzymes have garnered attention for their potential to degrade high-polymer materials. Enzymatic catalysis offers a unique solution to the challenges posed by plastics, ushering in a new era of environmental remediation.

The journey of PET degradation began with the groundbreaking discovery of the first PET-degrading enzyme in Thermobifida fusca by Müller and colleagues in 2005. Subsequently, multiple research teams have explored PET-degrading enzymes from various microbial sources, leading to remarkable advancements in the field.

Understanding the detrimental effects of PET on marine life and human health, our team embarked on a mission to address microplastic pollution. We undertook the construction of pET23b-INP-PETase engineered bacteria sourced from the Osaka fungus. Leveraging surface display technology, we harnessed the power of PETase, showcasing it on the cell membrane to facilitate PET degradation in the extracellular environment.

Our contributions extend to the realm of synthetic biology. We proudly introduce novel components, including the INP-PETase fusion part and the INP-linker-CBD fusion part. Additionally, we have updated the content of the CBD (Cellulose-Binding Domain) existing part. These advancements are poised to empower future IGEM teams seeking innovative solutions to combat microplastic pollution.

Our work emphasizes the importance of collaboration within the IGEM community and the continual evolution of synthetic biology. By sharing our knowledge and contributions, we aim to inspire and guide future teams in their pursuit of groundbreaking solutions. Together, we can work towards effective plastic waste reduction, protecting marine ecosystems, and ensuring human health.

In conclusion, we extend our heartfelt appreciation to the IGEM community for fostering an environment of scientific exploration and cooperation. Together, we possess the capacity to shape a brighter, more sustainable future for our planet. It is a call to action, a commitment to safeguarding our world for generations to come.