iGEM Attributions

iGEM Competition 2023: Attribution Form

Team: 2023 WageningenUR

Team Member Contributions

Name	Role	Tasks	Specific Tasks
Zhongyuan Lu	Student	Investigation, Software, Writing, Visualization	Head of Design Team; Creation of an agent-based model for colonisation behaviour, survival, and competitiveness of our engineered bacterium in the soil. Documentation of our iGEM journey with pictures and participation in the creation of the promotion video.
Bartlomiej Szeptycki	Student	Investigation, Background Research, Public Engagement, Software, Writing	Member of Human Practices-Team. Creation of a neuronal network to predict frost events from recurring data from weather sensors and modeling of FT-concentration in plants according to the current and predicted weather. Social-media manager.
Lorenzo da Silva Saraiva	Student	Analysis, Investigation, Notebook/record keeping, Wiki Coding, Safety	Lab safety manager and member of the design team. Growth and protein production of P. fluorescens in different temperatures and under the control of several promoters, respectively. Implementation of a LAMP detection system in P. fluorescens. Contribution to Wiki-coding and design tasks, including the logo, business card, and poster creation.
Simon Hendriks	Student	Investigation, Fundraising, Public Engagement, Writing	Head of the human practices team. Study on the control of flowering time of A. thaliana by several flori- and antiflorigens. Contribution to the acquisition of sponsors, contact with (dutch) cherry farmers and scientists.
Agata Wieremiejczuk	Student	Investigation, Notebook/record keeping, Fundraising, Writing	Secretary and member of the human practices team. Conducted studies concerning off- and on-kill switches for Pseudomonas fluorescens. Main contributions in the safekeeping of information from meetings within the team, supervisors and stakeholders.
Bas Lipsch	Student	Conceptualization, Investigation, Public Engagement, Software, Wiki Coding, Writing	Member of the design team. Creation of a model, based on differential equations to predict gene expression and growth of a single cell under certain conditions (e.g. temperature), as well as the design of the wiki-page.
Johannes Heisterberg	Student Leader	Conceptualization, Investigation, Project Administration, Visualization, Writing	Team captain. Studies on the interaction of P. fluorescens with A. thaliana (attachment and secretion of proteins into root-epidermis). Creation of Design elements for the wiki and contributions to the promotion video.
Nico van Donk	Student	Investigation, Fundraising, Public Engagement, Writing	Member of the human practices team. Implementation of a quorum-sensing and root exudate-dependent protein production in P. fluorescens. Cooperation with stakeholders, including sponsors, farmers and scientists.
Markus Bisschops	Secondary PI	Conceptualization	Assist in the supervision of the wet-lab and modelling subprojects.
Enrique Asín García	Instructor	Conceptualization, Background Research	Supervision of Nico van Donk working on quorum-sensing and root exudate-dependent protein production in P. fluorescens. Help to the human outreach team during brainstorming sessions.
Lyon Bruinsma	Instructor	Conceptualization, Background Research	Supervision of Lorenzo da Silva Saraiva characterizing the growth and protein production of P. fluorescens in different temperatures and under the control of several promoter. Advice about the implementation of a LAMP detection system. Help with preparation of the final presentation video.
Antoine Raynal	Instructor	Conceptualization	Assist in the supervision of the wet-lab subprojects.
Sarah DAdamo	Secondary PI	Conceptualization	Help with the conceptualization of the project and assessment of it's feasibililty.
Henk van Lingen	Instructor	Conceptualization	Supervision of the modelling subprojects. Advice on statistical analysis used in the models.
Vittorio Rainaldi	Instructor	Conceptualization, Background Research	Supervision of Simon Hendriks in the research on the control of flowering time of A. thaliana
Charlotte Koster	Instructor	Conceptualization, Public Engagement, Background Research	Supervision of Johannes Heisterberg characterizing the interaction of P. fluorescens with A. thaliana. Help to the human outreach team during brainstorming sessions.
Ricardo Villegas	Instructor	Conceptualization, Background Research	Advice to Agata Wieremiejczuk on the CRISPR-Cas system.
Matic Kostanjek	Instructor	Conceptualization	Assist in the supervision of the wet-lab subprojects.
Miguel paredes barrada	Instructor	Conceptualization	Supervision of Agata Wieremiejczuk working on the implementation of kill-switches in P. fluorescens.
Robert Smith	Primary PI	Conceptualization	Supervision of the entire team throughout the project. Supervision of the modelling subprojects.
Nico J Claassens	Primary PI	Conceptualization	Supervision of the entire team throughout the project. Supervision of the wet-lab subprojects.

External Contributions

Name	Institution Type	Relationship To Team	Tasks	Specific Tasks
Prof. Dr. Ir. Richard Immink	Academic	External Advisor	Conceptualization, Background Research	- Shed light on the complex world of plant flowering pathways. - Steered us in the direction of using the PEBP-gene family as our main target for anti-florigens. - Advised us in the design and set-up of our in vivo plant experiments, to use Arabidopsis thaliana as a test subject.
Dr. Wouter Kohlen	Academic	External Advisor	Conceptualization, Background Research	Advised us in the design and set-up of our in vivo plant experiments, to get as much data out of the limited amount of experiments that we could perform.
Prof. Emer Dr. Steven Lindow	Academic	External Advisor	Conceptualization, Background Research	- Communication about GMO’s to the outside world is delicate but very important. - Injection in the root cells is tricky and needs sufficient designing and engineering.
Dr. rer. agr. Klaus-Peter Götz	Academic	External Advisor	Conceptualization	Scientific help: Transport in the phloem of a tree can be sufficiently fast. - There is evidence of peptides produced in the roots that are transported to the shoot apical meristem.
Jochem van Dijke	Industry	External Advisor	Conceptualization, Background Research	Dutch Fruit Farmer -Helped us to get familiar with general orchard cultivation practices. - Current frost damage counter measures are greatly dependent on the intuition of the farmer on when to apply it. - Current frost damage counter measures all have significant disadvantages.
Herman Capel	Industry	External Advisor	Conceptualization, Background Research	Fruit farmer at Capel Fruit: - Enlightened us about the general trends in frost damage. - Spreading the harvest across an orchard is not only interesting to spread the risk of frost damage but also to reduce time pressure during harvest season. - Current frost damage countermeasures all have significant disadvantages.
Johan Sonneveld	Industry	External Advisor	Conceptualization	Fruit consultant at Fruitconsult B.V. - The year 2023 was really bad for cherry farmers in the Netherlands. - The most popular cherry tree species (Cordia) in the Netherlands is especially susceptible to frost damage. - Helped us with shaping and finalizing our application strategy. - Farmers will be very interested if the flowering delay is proven to be effective.
Jeannet Mulder	Nonprofit	External Advisor	Conceptualization	Helped us in the development and distribution of an online survey for farmers.
Dr. Cécile van der Vlugt-Bergmans	Government	External Advisor	Conceptualization	Risk assessor GMO at the GMO office of the RIVM (Dutch National Institute for Public Health and the Environment) - There is a strong need for a detection method for our product - Product behavior characterization is the number one priority when thinking about the next steps in research and commercialization.
Dr. Cynthia van Rijn	Government	External Advisor	Conceptualization	Risk assessor GMO at the GMO office of the RIVM (Dutch National Institute for Public Health and the Environment) -Product behavior characterization is the number one priority when thinking about the next steps in research and commercialization.
Prof. dr. Paolina Garbeva	Industry	External Advisor	Conceptualization	Senior researcher at the Netherlands Institute of Ecology (NIOO-KNAW) Prof. dr. Paolina Garbeva's research is focused on studying the mechanisms of microbial communication and plant-microbe interactions.
Sohini Deb	Academic	Sponsor	Background Research, Other	Donation of the Pseudomonas fluorescens etHAn strain from Copenhagen University for the investigation of protein injection from bacteria to plant.
Helmholtz Institute for RNA-based Infection Research, Helmholtz Centre for Infection Research, Würzburg, Germany.	Academic	Sponsor	Other	Provided with the SuCas12a2 nuclease used in the project.
WUR Student Challenges	Academic	Sponsor	Other	Provided us with financial support.
SnapGene	Industry	Sponsor	Other	Provided us with the licenses for the SnapGene software.
New England Biolabs inc.	Industry	Sponsor	Other	Provided us with in-kind support in the form of lab reagents.
Vlag Graduate School	Academic	Sponsor	Other	Provided us with financial support.
Integrated DNA Technologies IDT	Industry	Sponsor	Other	Provided us with in-kind support in the form of free gene fragments.
Centre for Living Technologies	Academic	Sponsor	Other	Provided us with financial support.
Wageningen University & Research	Academic	Sponsor	Other	Provided us with financial support, laboratory equipment, and space to work and conduct experiments.
Bioké	Industry	Sponsor		Provided us with in-kind support.
QIAGEN	Industry	Sponsor		Provided us with in-kind support.
Twist Bioscience	Industry	Sponsor		Provided us with in-kind support.

Project Timeline

Project Activity

Start Date

End Date

Total Time (weeks)

1. Recruit Team Members

2. Development of Project Plan

3. Wet Laboratory Activities

4. Dry Laboratory Activities

5. Building the Team Wiki