Our team has engineered E. coli with a plasmid that integrates the three genes that enable resveratrol synthesis, plus the RFP PPOR gene. This new part has been designed and included in the iGEM registry, and is a Level 2 Golden Gate module, which therefore includes all the Level 1 modules required for resveratrol biosynthesis in E. coli.
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K4671007 | Composite | pJUMP49-2A containing transcriptional units for TAL, 4CL, STS and PPOR | Lucas Castellani | 21273 bp |
Parts
The following Level 1 parts of iGEM registry are included:
- BBa_J428361: pJUMP49-2A SpecR Type IIS Level 2 vector for A modules
- BBa_K4671004: pJUMP-TAL
- BBa_K4671005: pJUMP-4CL
- BBa_K4671003: pJUMP - STS
- BBa_K4671006: pJUMP- PPOR
Last four of the above-mentioned parts are composed of the following basic parts:
- BBa_J428341: pJUMP29-1A KanR Type IIS Level 1 vector for A modules
- BBa_J435350: AB_T7_lacO
- BBa_J435315: BC_BCD2
- BBa_J435361: EF_T7term
- BBa_K4671002 (TAL from Saccharothrix espanaensis) , BBa_K4671001 (4CL from Arabidposis thaliana), BBa_K4671000 (STS from Vitis vinifera) or BBa_J435087 (pporRFP).
All information on the genes included, as well as the design of the part, is available in the "Engineering" section.
This new part may develop many advantages for future IGEM teams and researchers interested in Resveratrol biosynthesis in E. coli. Until now, the bioproduction of this antioxidant had to be carried out by introducing each of the modules into the bacteria to form a final construct. However, this plasmid would allow immediate capacity for synthesis by simply transforming E. coli. Thus, research into this molecular potential can be accelerated and enhanced through the use of this part, which will hopefully contribute to further scientific development in iGEM. In addition, as included and described in "Engineering", gene expression must be induced with IPTG. It should also be noted that the design has been designed specifically for this organism and should not be introduced into other microbial species.
At the moment, we have not been able to implement the final construction in bacteria, as we have only managed to clone individual L1s. However, we are continuing the work in the lab until the Jamboree, and we hope to soon achieve the final construction presented on this page. Our objective is to test its functionality, analyse and quantify the bioproduction obtained with the designed part.