Read about our parts contributions and a new promoter library with a wide range of activity in methanotrophs.
The Henard Library is the mutagenized set of promoters that the 2023 iGEM team, as part of the Henard lab managed to create by introducing a small range of mutations to the methane monooxygenase promoter which is highly active in methanotrophs. We were able to isolate mutated promoters with high, low, and medium promotion efficiency in bath and e.coli. We hope that this new library, which fills the gap left by the lack of dynamic range of the Anderson series in methanotrophs, will be useful to any future iGEM teams that want a promoter to suit a specific rate of expression. We also hope it can inspire future iGEM teams to work with methanotrophs and realize their potential as biocatalysts.
The promoters were created through mutagenesis of the wildtype PpmoC2 promoter in Bath, leading to the creation of five unique promoters that perfectly bridge the gap between the p119 promoter and p104 in the anderson series (the bridged gap is specifically for e.coli), together with the anderson series promoters we managed to achieve a dynamic range of promoters that can be useful in the constant and non harmful expression of desired genes in bath and e. coli. Note that the native promoter was isolated from bath origially so the activity of the Henard series is a little higher in bath. The mutated promtoers are as listed, with the sequences available on the iGEM registry.
These promoters and our revised research on the activity of the Andreson series in e. coli, bath, and Ob3b constitute our contributions to future iGEM teams.