This page details who did what during our project.
Etash was responsible for cloning all of the Anderson Promoters into the respective vectors using Gibson Assembly so that we could transform them into competent cells and e. coli to measure fluorescence through the promotion of the RFP gene. He is responsible for media preparation, mating, and minipreps for sequencing. A large amount of the original legwork was done by Etash and he will continue to be responsible for crucial parts of the project.
Logan was responsible for conducting basic procedures for the research, involving preparation of media, Mini-Prep of plasmid DNA from bacterial cultures, transformation of DH10b and S17 E. coli, and the mating and streaking of Bath and OB3b methanotrophs with promoters 115, 116, 117, and 118. A large part of the work was also carrying out Q5 PCR tests, gel confirmations, Nanodrop testing, and DNA purification, as well as doing minor lab tasks like washing glassware and refilling plastic items (pipette tips and microcentrifuge tubes).
Mahith was responsible for conducting the preparation of some of the original Anderson series plasmid vectors, preparation of media, transformation of Dh10b's and s17 competent cells, mating and isolation into m. capsulatus bath and OB3b methanotrophs, promoters 100-107 of the Anderson series. Group work consisted of PCR and qPCR for confirmatin of results, nanodrop and gels for further confirmation, DNA clean-up/purification, lab chores, video editing, and sample creation for use in the flourimeter to obtain quantitative experimental results.
Elle was responsible for a large portion of the wiki, an additional external Wix site, media preparation, lab chores, transforming and mating promoters 106, 107, 110, and 114 as well as sample preperation/data entry for most of the flourescence assays. She was also responsible for some of the initial work regarding the mutagenesis portion of the project, but a large amount of the creation of the promoter library was done with ample help from more technically experienced lab members.