As a part of our endeavour to improve the efficiency of MICP and thus reduce the majority of carbon emissions from clinker formation in cement. We used an existing variant of carbonic anhydrase to see if we can improve the catalytic efficiency of MICP. Though we were able to characterise it we did not get as far as to confirm function in MICP process. http://parts.igem.org/Part:BBa_K4758000

The bulk of our software system and its constituent subsystems can be found in a GitHub repository. The central idea regarding automation is to provide an accurate and precise alternative to assays ensuring high-throughput results.

To replicate the integrated setup, a README.md file has been provided on the home page. This file includes the instructions on how to set up each subsystem (namely the xArm6, OT2) as well as a subsequent integrated set up. The programs specific to each subsystem (as well as the Jupyter Notebook to run the protocols) are in a separate folder named according to each subsystem. Links provided in the README.md file also contain links for troubleshooting with regards to each subsystem.

Plate reader results confirm the visual inspection, displaying higher absorbance at higher urease concentration.

Each program contains comments regarding its purpose. With regards to the Carbonic Anhydrase assay set up in the OT2, procedural outline surrounding the cell lysate prework preceding the assay is outlined in the overview alongside steps that require manual involvement. With regards to the specific processes surrounding assay automation, the list of reagents used as well as their respective concentration (in molarity) has been listed. Furthermore, all other factors affecting the outcome of the experiment (I.e. measurement intervals) are listed and should be followed to ensure accurate replication of result. Subsequently, the location of each labware has been labelled and can be varied according to the needs of the future teams. All intermediate steps have been provided as well.