Attributions

Table 1 : Team Member Contributions

Name Role General Tasks Specific Tasks
Ghadir Rizk Student Leader Conceptualization, Background Research, Project Administration, Fundraising, Public Engagement, Entrepreneurship, Visualization, Writing. (1) Conceptualization: participated in creating action plans for the project and setting goals; (2) Background Research: review of materials to consolidate the theme; (3) Project Administration: coordination of the team's broad front, helping to organize meetings, define goals, document presentations at events and contact with public bodies; (4) Fundraising: participation in sponsorship and financing; (5) Public Engagement: helping to create educational materials for the community and tools for dialogues in the region; (6) Entrepreneurship: creation of entrepreneurial materials, participation in events, contacts with companies and public institutions; (7) Visualization: creation of project name and logo; (8) Writing: writing team description pages, partnerships, attributions on the wiki and reviewing the complete material.
Anali Vitória de Queiroz Student Leader Project Administration, Fundraising, Entrepreneurship, Writing. (1) Project Administration: Managing and coordinating project resources, schedules, and tasks to ensure timely completion and alignment with set objectives. (2) Fundraising: Leading efforts to garner financial support for the project through grant applications, events, and outreach to potential donors or sponsors. (3) Entrepreneurship: Identifying and acting on opportunities to transform project outcomes into viable business ventures or innovative solutions, ensuring sustainability and impact. (4) Writing: Crafting clear and coherent content, such as reports, articles, and proposals, to communicate the project's findings, strategies, and significance to various audiences.
Jamily Brito Dorado Student Leader Analysis, Conceptualization, Background Research, Investigation, Data Curation, Notebook, Project Administration, Writing, Safety. (1) Analysis: analysis of experiment results from our team, other iGEM teams and literature results for experimental design; (2) Conceptualization: brainstorming ideas for the project theme, project experiments and participated in developing the wet lab activities calendar; (3) Background Research: reviewing and reading articles, guidelines, protocols, different iGEM teams wikis and documents to substantiate and trace the Wet Lab activities; (4) Investigation: planning and projecting experiments, executing experiments, data collection, results analysis and lab maintenance; (5) Data Curation: annotating protocols and maintaining research and experiment data for documentation; (6) Notebook: keeping record of all laboratory activities through written records, videos and photos, maintaining laboratory notebook; (7) Project Administration: coordinating and managing the wet lab activities, scheduling meetings, planning experiments and activities, executing experiments, updating all team members of the progress while keeping a safe and healthy environment for the wet lab members; (8) Writing: writing the Experiments and Notebook wiki pages, reviewing documents such as research documents and protocols, reviewing all wet lab related wiki pages; (9) Safety: ensure compliance with the safety guidelines and iGEM requirements.
Julio Cesar Riveros Cardus Student Leader Analysis, Conceptualization, Background Research, Investigation, Data Curation, Project Administration, Wiki Coding, Visualization, Writing, Safety. (1) Analysis: Evaluating experiment results from our team, other iGEM teams, and existing literature to inform experimental design; (2) Conceptualization: Reviewing articles, guidelines, protocols, and documents from different iGEM teams to support and guide dry lab activities; (3) Background Research: Examining relevant materials to solidify our project's theme. (4) Investigation: Planning and conducting experiments, collecting data, analyzing results, and developing structural models; also, implementing experimental designs. (5) Data Curation: Annotating protocols and overseeing the organization and management of research and experiment data for documentation. (6) Project Administration: Coordinating and overseeing dry lab activities, scheduling meetings, planning experiments and tasks, conducting experiments, and keeping all team members informed of progress. (7) Wiki Coding: Providing user training, documenting project details, presenting data, formatting content, and organizing information. (8) Visualization: Creating the project name and logo. (9) Writing: Drafting team model and safety pages. (10) Safety: Designing and formulating a killswitch for safety measures.
Lucas Ovelar Student Leader Analysis, Conceptualization, Background Research, Investigation, Data Curation, Notebook, Project Administration, Visualization, Writing, Safety. (1) Analysis: Evaluating and interpreting data and results from scientific articles and other iGEM teams, to draw conclusions and provide insights; (2) Conceptualization: Brainstorming and formulating the foundational ideas and hypotheses that guide the project's direction and objectives; (3) Background research: Conducting a thorough review of existing literature about microalgae, design, and enzymes, to understand the current state of knowledge and identify gaps or opportunities; (4) Investigation: Actively exploring, experimenting in the laboratory, and gathering information to validate hypotheses or answer specific project questions. (5) Data Curation: Organizing, cleaning, and ensuring the quality and integrity of data collected. (6) Notebook: Maintaining a detailed and organized log of all experiments, observations, and results. (7) Project Administration: Overseeing and coordinating the experiments. Also coordinating the connection of DryLab and WetLab front. (8) Visualization: Creating graphical representations of information and the team branding. Production of the promotional video. (9) Writing: Drafting reports, protocols, and documentation to communicate the project's. Writing wikis pages. (10) Safety: Ensuring that all project activities adhere to established safety protocols and guidelines, minimizing risks to team members and the environment.
Grecia Antonella Nina Flores Student Leader Analysis, Conceptualization, Project Administration, Public Engagement, Visualization, Writing. (1) Analysis: Conducted a literature review to guide the strategic planning of our Human Practice project; (2) Conceptualization: identified key issues that helped shape the activities developed under the project; (3) Project Administration: Participated in the management, coordination, planning, and execution of all activities related to Human Practice; (4) Public Engagement: Designed and implemented educational activities and outreach materials to involve the local community in environmental education and synthetic biology; (5) Visualization: Designed and edited instructional and educational videos that were used to convey key concepts related to our human practice project; (6) Writing: Contributed to the writing of specific sections of the wiki, promotional and planning materials for the Human Practices front.
Julio Barbosa Student Conceptualization, Background Research, Visualization, Writing (1) Conceptualization: Participated in the creation of action plans for the development of the project and the definition of objectives; (2) Background Research: Review of bibliographic materials to consolidate and achieve the objectives proposed in the project; (3) Visualization: creation of educational material for teaching environmental education; (4) Writing: writing pages for the design of the wiki, on the human practices front.
Ivan Mateo Spitia Student Analysis, Background Research, Writing. (1) Analysis: participated in building documents for the wet lab activities based on the analysis of data generated by different iGEM teams in the wet lab front; (2) Background Research: reading articles to build documents that were needed for wet lab activities; (3) Writing: participated in writing the ManuAlgae and the Protocols wiki pages.
Thaynara Cristina de Carvalho Student Analysis, Background Research, Writing, Investigation. (1) Analysis: participated in building documents for the wet lab activities based on the analysis of data generated by different iGEM teams in the wet lab front; (2) Background Research: reading articles to build documents that were needed for wet lab activities; (3) Writing: participated in writing the ManuAlgae and the Protocols wiki pages. (4) Investigation: Experimenting in the laboratory doing culture medium and helping to organize the laboratory inventory.
Nájua El Kadri Student Analysis, Conceptualization, Background Research, Investigation, Data Curation, Visualization, Writing, Social Media, Wiki Coding, Safety. (1) Analysis: Verification of previous modeling, hardware, experiments, and implementations made by other teams; critical analysis of literature data that could contribute to the team's model and feasibility analysis of the constructed model and implementation; (2) Conceptualization: active participation in defining the project theme; developing implementation and modeling ideas, as well as strategies for formulating experiments with the available resources; (3) Background Research: reading and collecting data from articles regarding the chassis, target plastic, photobioreactors, design parts, modeling and data from sanitation books and national surveys in addition to laboratory protocols; (4) Investigation: planning, collecting specific data, and analyzing results into the development of kinetic models and implementation, in addition to deepening the design of photobioreactors and metabolic functioning of microalgae; (5) Data Curation: management of variables and parameters of kinetic modeling; organization in tables, documents, and periodic updates; data cleaning and preparation from interviewees of Human Practices project for subsequent manipulation; (6) Visualization: visualization of data from interviewees from the Human Practices project in graphs; modeling and implementation illustrations; (7) Writing: writing of kinetic modeling, implementation, and education wiki pages, in addition to content review; (8) Social Media: support for social networks, in design, marketing, and engagement strategies, in addition to content creation; (9) Wiki Coding: presenting data, formatting content, and organizing information in GitLab. (10) Safety: research on microalgae biocontainment for implementation and critical analysis regarding killswitch feasibility.
Micael Baruch Student Analysis, Conceptualization, Background Research, Investigation, Data Curation, Visualization, Writing. (1) Analysis: Assessing experiment results from our team, other iGEM teams, and existing literature to inform experimental design. (2) Conceptualization: Reviewing articles, guidelines, protocols, and documents from different iGEM teams to guide dry lab activities. (3) Background Research: Examining relevant materials to solidify our project's theme. (4) Investigation: Conducting experiments, collecting and analyzing data, developing kinetic models, and implementing experimental designs, including the selection of biological components for our design. (5) Data Curation: Annotating protocols and overseeing the organization and management of research and experiment data for documentation. (6) Writing: Creating team model pages.
Isabela Dal Moro Student Conceptualization, Public Engagement, Writing. (1) Conceptualization: Participated in the creation of action plans for the development of the project and the definition of objectives; (2) Public Engagement: Designed and implemented educational activities and outreach materials to involve the local community in environmental education and synthetic biology; (3) Writing: Contributed to the writing of specific sections of the wiki, promotional and planning materials for the Human Practices front.
Leticia Sandri Student Conceptualization, Public Engagement, Writing. (1) Conceptualization: Helped to carry out practical activities in schools; (2) Public Engagement: Helped implement educational activities and publicity materials; (3) Writing: Contributed to the writing of specific sections of the wiki for the Human Practices front.
Maria Eduarda Catalan Student Conceptualization, Public Engagement, Writing. (1) Conceptualization: Participation in the creative process of educational tools for the Human Practice front project; (2) Public Engagement: preparation of theoretical and practical material for games aimed at teaching Synthetic Biology; (3) Writing: participation in writing the education wiki pages.
Gabriela Santana Student Conceptualization, Public Engagement, Writing. (1) Conceptualization: participation in the creative process of educational tools for the Human Practice front project; (2) Public Engagement: preparation of theoretical and practical material for games aimed at teaching Synthetic Biology; (3) Writing: participation in writing the education wiki pages.
Elizandra Camilo Student Fundraising, Writing (1) Fundraising: assistance in preparing the necessary documentation to obtain sponsorship and funding; (2) Writing: Participation in writing the Wiki funding page.
Henrique Rubino Martins Student Project Administration, Public Engagement, Writing, Social Media. (1) Public Administration: Oversees the coordination and management of communication-related resources, ensuring that the team's messaging aligns with the project's objectives. (2) Public Engagement: Leads initiatives to connect with the broader community, stakeholders, and the general public. (3) Writing: Crafts clear and coherent content in Wiki, to communicate the project's findings. (4) Social Media: Manages and curates content for online platforms such as Instagram, Facebook, and LinkedIn. He ensures the team engages with a wider audience, disseminates project updates, and fosters a digital community around the project's themes and objectives.
Sophia Politis Ribeiro Student Public Engagement, Writing, Social Media (1) Public Engagement: Leading outreach efforts to connect with the community and stakeholders, aiming to educate, inform, and gather input about the project's goals and results, by social media platforms, like: Instagram and Facebook. (2) Writing: Producing well-structured content, from articles, to effectively convey the project's discoveries and significance to diverse readers; (3) Social Media: Conceptualizing contentont for Instagram, Facebook, LinkedIn and Wiki, to share project milestones, engage with followers and create a vibrant digital presence around project themes.
Julia Bahe Cardoso Student Public Engagement, Writing, Social Media (1) Public Engagement: Spearheading community outreach via platforms such as Instagram and Facebook to foster connections with the community and key stakeholders. (2) Writing: Crafting articulate content, particularly articles, to effectively communicate the project's findings and their importance to a wide range of readers. (3) Social Media: Conceptualizing and generating content for platforms including Instagram, Facebook, LinkedIn, and Wiki.
Cristian Antonio Rojas PI Conceptualization, Background Research, Project Administration, Investigation, Safety, Supervision (1) Conceptualization: Help in creating and consolidating the project theme; (2) Background Research: Reading and reviewing scientific materials for the laboratory front. (3) Project Administration: Coordinating the team and setting tasks for the front of the wet lab; (4) Investigation: Performing laboratory experiments; (5) Safety: Collaboration to ensure biosafety in laboratory activities, review of materials, and alignment with competition guidelines and institutional regulations; (6) Supervision: Supervision of laboratory activities and material support.
Samuel Chagas Advisor Conceptualization, Supervision (1) Conceptualization: Help with creating ideas for the project's dry lab front and consolidating the theme; (2) Supervision: Supervising the team's modeling activities.
Giulio Mendes Bratz Advisor Conceptualization, Supervision (1) Conceptualization: Help in developing and creating ideas for the project theme; (2) Supervision: Supervision and support for the deliveries requested for the competition.
Matheus Araujo dos Santos Advisor Conceptualization, Supervision (1) Conceptualization: Help with creating ideas for the project's wet lab front and consolidating the theme; (2) Supervision: Supervision of laboratory activities and material support.
João Molino Advisor Conceptualization, Supervision, Investigation (1) Conceptualization: Help with creating ideas for the project's wet lab front and consolidating the theme; (2) Supervision: Supervision and assistance in the project's laboratory activities; (3) Investigation: Provided us with a plasmid.

Table 2 : External Contributions

Name Institution Type Relationship to Team General Tasks Specific Tasks
SANEPAR Industry External Advisor Investigation Provided data and information on the use of microorganisms in sewage treatment plants
Itaipu Binacional Industry Sponsor Fundraising Awarded a grant to support our team.
Eliana Navarro Academic External Advisor Investigation Participation in interviews conducted by Human Practices staff to collect data on education in Latin America
Karina Pacsi Academic External Advisor Investigation Participation in interviews conducted by Human Practices staff to collect data on education in Latin America
Vera Bana Academic External Advisor Investigation Participation in interviews conducted by Human Practices staff to collect data on education in Latin America
Mariana Gravina Academic External Advisor Investigation Participation in interviews conducted by Human Practices staff to collect data on education in Latin America
Consuelo Navarrete Academic External Advisor Investigation Participation in interviews conducted by Human Practices staff to collect data on education in Latin America
Marli Queiroz Academic External Advisor Investigation Participation in interviews conducted by Human Practices staff to collect data on education in Latin America
Ronaldo Adriano Ribeiro Academic External Advisor Investigation Discussed and guided the members of the human practices on how to structure the interviews with elementary school teachers.
Cleto Kaveski Peres Academic External Advisor Investigation Shared materials, documents, and information on the morphophysiological characteristics of microalgae.
Patricia Justen Da Silva Academic External Advisor Investigation Participation in interviews conducted by Human Practices staff to collect data on education in Latin America
Edith Verón Academic External Advisor Investigation Participation in interviews conducted by Human Practices staff to collect data on education in Latin America
Isac José da Silva Filho Academic External Advisor Investigation Donation of Chlamydomonas reinhardtii strains to enable the implementation of the laboratory project.
Vitor Marchesan Academic External Advisor Investigation Collaborated by sending materials and sharing information on the construction of photobioreactors.
Maura Harumi Sugai Guerios Academic External Advisor Analysis Addressed what was missing in the model, such as the rate of expression or secretion of the protein, and also addressed the possibility of setting oxygen equal to zero, to simplify the model.
Wasim Syed Academic External Advisor Analysis Reviewed the project's structural modeling material.
Gustavo Bruschi Academic Collaborator Background Research Background Research: reading and reviewing scientific materials for the laboratory front.
Livia Seno Camargo Academic External Advisor Investigation Donation of Chlamydomonas reinhardtii strains to enable the implementation of the laboratory project.
Marcus Vinicius Xavier Senra Academic External Advisor Investigation Shared useful articles to develop strategies for the Chlamydomonas reinhardtii cloning method.
Allison Borba Cabral Academic Collaborator Wiki Coding Writing code to enable the development of Wiki web pages in GitLab.
Bernie Rolando Reyes López Academic Collaborator Investigation Executed and supported the laboratory experiments carried out by the Wet Lab front.
Marcel Valverdes Academic Collaborator Logistic Helped in the logistics to obtain donations of Chlamydomonas reinhardtii strain.
Wesley Luzetti Academic External Advisor Investigation Provided antibiotic for the selection of transformed microalgae.
João Vitor de Paula Academic Collaborator Wiki Coding Writing code to enable the development of Wiki web pages in GitLab.
Raí Pacheco Other Collaborator Video production Helped in the development of videos for Human practices.
Rosario Calabró Other Collaborator Video production Voice-over for the promotional video of the project.
Giulia Knop Academic Collaborator Art production Creation of art for the educational activities of the Human Practices front.
Bruno Zepeda Academic Collaborator Writing Assisted in the orthographic adjustment of the pages of Human Practices in the Wiki.
Natan Reis Azarias Academic Collaborator Video production Translation into the Brazilian language of signs of the audiovisual materials of the environmental educational project of the Human Practices front.
Roberto Bernal Mazacotte Academic Collaborator Video production Translation into the Brazilian language of signs of the audiovisual materials of the environmental educational project of the Human Practices front.
Angel Gabriel Vera Insfran Academic Collaborator Video production Help to translate the educational video script in Guaraní
Luis Enrique Mendonza Martens Academic Collaborator Video production Help to translate the educational video script in Guaraní
Gissella Madaly Martínez Silva Academic Collaborator Video production Help to translate the educational video script in Guaraní
Gladis Flores Carlos Other Collaborator Video production Help to translate the educational video script in Quechua
Vicentina Huamanquispe Saico Other Collaborator Video production Help to translate the educational video script in Quechua

Table 3 : Project Timeline

Project Activity Start Date End Date Total Time
Recruit Team Members 04/07/2022 22/08/2022 6 weeks
Development of Project Plan 22/08/2022 16/12/2022 16 weeks
Dry Laboratory Activities 01/02/2023 09/10/2023 35 weeks
Wet Laboratory Activities 28/07/2023 05/10/2023 11 weeks
Building the Team Wiki 21/08/2023 10/10/2023 7 weeks