NOTEBOOK

Weekly Notebook

6/19 - 6/23

Bacterial transformation training

  • Plasmid transformation
  • Cell pre-culture
  • Plasmid extraction
  • PlaLarge culture

6/26 - 6/30

  • Electrodeposition chitosan hydrogels
  • Prepare plasmids for sequencing

7/10 - 7/14

  • Electrode cleaning training
  • Cyclic Voltammetry training

7/17 - 7/21

  • Chitosan hydrogel: 1% v/v acetic acid + 1% w/v chitosan
  • Chitosan hydrogels with purple dye electrodeposited onto gold electrodes with chronoamperometry
  • Soaked in pH 7 and pH 3 for 30 minutes
  • Dye concentration was not high enough to measure

7/24 - 7/28

  • Assembling enzyme fuel cells training
  • Enzyme concentration: 1.13 mg/mL
  • Power Test with GDH & ferricyanide

7/31 - 8/4

  • Repeat power tests with higher concentrations of enzyme (7 mg/mL)

8/7 - 8/11

  • Repeat power tests with higher concentrations of Ketjen black
  • Connected enzyme fuel cells in series to increase power
  • Tested BLE device
  • Gel Electrophoresis of PQQGDH + pTRC vector

8/21 - 8/25

  • PQQGDH expression
  • Pyocyanin power testing

8/28 - 9/1

  • PQQGDH transformation with PTRC vector into BL21 DE3 E. coli

9/4 - 9/8

  • Pre-culture
  • Overnight large culture
  • Large culture harvest
  • DC protein assay

9/11 - 9/15

  • Power Test: PQQGDH & Ferricyanide
  • PQQGDH protein purification with Histag affinity column
  • Pyocyanin arrives!!

9/18 - 9/22

  • PQQGDH protein purification with cation affinity column
  • Power Test: PQQGDH & Pyocyanin

9/25 - 9/29

  • Power test results analysis

10/2 - 10/6

  • Activity testing for pyocyanin and PQQ

10/9 - 10/12

  • Wiki finalization