PQQGDH

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Our contribution is the addition of a new part to the iGEM registry (Part:BBa_K4893001), pyrroloquinoline quinone glucose dehydrogenase (PQQGDH). This enzyme can be used by future iGEMers working with electron transfer reactions.

A. calcoaceticus derived PQQGDH is a dimeric enzyme which is recombinantly produced using E. coli as a host microorganism. It is responsible for oxidizing glucose into glucono-δ-lactone which is then hydrolyzed into gluconic acid. In PQQGDH holo form, it possesses the PQQ cofactor that gives its redox abilities. This enzyme has been most widely used for glucose sensing applications. In our project we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa detection.

This enzyme has been most widely used for glucose sensing applications. In our project we present a new application of this enzyme in pyocyanin sensing for P. aeruginosa applications.

References

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Sode K, Ootera T, Shirahane M, Witarto AB, Igarashi S, Yoshida H. Increasing the thermal stability of the water-soluble pyrroloquinoline quinone glucose dehydrogenase by single amino acid replacement. Enzyme Microb Technol. 2000 Apr 1;26(7):491-496. doi: 10.1016/s0141-0229(99)00196-9. PMID: 10771051.

Igarashi S, Sode K. Stabilization of quaternary structure of water-soluble quinoprotein glucose dehydrogenase. Mol Biotechnol. 2003 Jun;24(2):97-104. doi: 10.1385/MB:24:2:97. PMID: 12746550.