Create a SENSE and TREAT system for P. aeruginosa infections in skin wounds.
is a multidrug-resistant bacterium
that can cause diseases like pneumonia and meningitis.
In 2017 alone, 32,600 infections and 2,700 deaths were caused by P. aeruginosa.
Current detection methods take between
hours via swap samples and plate cultures.
By detection time, P. Aeruginosa has already spread and formed a biofilm, making it harder to eradicate.
We develop a rapid detection method for P. Aeruginosa
coupled with an immediate treatment mechanism
using a SENSE and TREAT design.
We build a pyocyanin biosensor, which detects P. aeroginosa via the pyocyanin biomarker
Pyocyanin's redox activity gives it the ability to carry electrons and transfer them to an electrode. We take advantage of this property and incorporate it into an electrochemical biosensor.
We target curcumin(a natural compound found in turmeric) as a treatment molecule, which acts as an antimicrobial and accelerates wound healing.
Our design uses a pH sensitive hydrogel to deliver curcumin to the site.
SENSE AND TREAT DESIGN
We use enzyme fuel cells (an anode and cathode placed in the fuel, a glucose solution) in our electrochemical unit.
Enzymes on the anode react with the glucose, resulting in electron transfer.
When Pyocyanin is present, the electrons transfer to the electrode, and a current is detected.
Meanwhile, the production of gluconic acid reduces the pH of the environment.
This affects the pH sensitive hydrogel that reacts by releasing curcumin and treating the wound!