Personal protective equipment
Risk group containment
Good laboratory practices
Biosafety levels
In the ongoing project, genetically modified organisms (GMOs) are employed to produce endolysins as an alternative to antibiotics, offering a novel approach to combat diseases and tackle antibiotic resistance. Within the laboratory context, maintaining safety protocols is of utmost importance. The laboratory infrastructure is designed to minimize potential risks associated with working with genetically modified organisms and to prevent potential release into the environment. By prioritizing robust safety measures, the research team aims to safeguard laboratory personnel and the broader community.
The experimental phase of the project was conducted in the laboratories on the second floor of the Engineering building at the Tecnológico de Monterrey, Campus Chihuahua (H. Colegio Militar Avenue 4700, Nombre de Dios, 31150 Chihuahua, Chih.). These laboratories are biosafety level 2 and are exclusively accessible to students who have completed the laboratory safety course offered by the institution.
During the experimental phase of the project, we were continuously assisted by either the laboratory technicians (M.S. Carolina Apodaca, & M.S. Ivonne Hernández) or our instructors (Ph.D. González Trevizo, & Ph.D. López), all of whom possess several years of experience in their respective fields. They have previously supervised and supported other iGEM Tec-Chihuahua teams in past years. We also were assisted by our instructors Eng. Daniela Oloño, Eng. Carolina Gajón, and Eng. Alejandro Rojo. Importantly, no experiments were conducted without the supervision of the aforementioned individuals.
As part of the preparation before commencing the experimental work in the laboratory phase of the project, team members completed and obtained certification in several online courses offered by the Public Health Agency of Canada for Biosafety e-Learning.1
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Reagents
After use, laboratory reagents and materials were stored in the laboratory warehouse for proper inventory management and safe usage.
Personal Safety
Our personal protection techniques like tying back hair, wearing a face mask, nitrile gloves, a lab coat, long pants, and close-toed shoes, were a fundamental aspect of our work, guaranteeing everyone's safety and maintaining experimental integrity.
Personal items
Non-disposable items were stored in their designated location and remained within its space, avoiding potential risks to team members and the campus community. Personal items that could contaminate, like necklaces and earrings, were not worn. The correct use of PPE was strictly upheld throughout laboratory activities.
Emergency protocol
For our personal safety, our laboratories provided us specific guidelines to know how to act in case of an accident on the workplace. This diagram includes the phone numbers of campus authorities. Also, it is placed over a first-aid kit. Additionally, our team made sure safety came first by making sure all emergency exit doors were clear and easy to use. We're not just focused on doing experiments well; we also want to create a culture where we're always watching out for potential issues and ready to handle anything that comes up
Hand safety
Consistent handwashing upon entry and exit and the use of 70% ethanol before and after wearing gloves ensured a clean and contamination-free environment. Additionally, we applied 70% ethanol to our hands before and after handling any laboratory samples, further enhancing our commitment to maintaining a sterile workspace. Furthermore, thanks to the Personal Protective Equipment course offered by the Public Health Agency of Canada, the team members responsible for the experimental phase of the project have adopted the correct procedure for removing nitrile gloves once their work in the laboratory is completed.
Sterile environment
Proactive sterilization of surfaces and equipment using 70% ethanol and burners upheld the laboratory's sanitary conditions. This dedicated commitment to hygiene protocols ensured the credibility and integrity of our experimental outcomes
Waste
Laboratory waste generally falls into four categories, each with its own disposal method. Proper disposal is crucial to prevent contamination and accidents. Solid biological waste goes into a yellow bag, while non-solid biological waste is placed in a sealed yellow bottle. Sharp objects and used electrophoresis gels are disposed of in a red plastic bottle. Any materials in contact with bacteria or other infectious agents are put into a red bag. Given our project involving bacteria, we mainly used red containers like bags and bottles for disposal.
Neutralization
Bacterial cultures and any associated debris were meticulously disposed of following the addition of sodium hypochlorite, a potent disinfectant, to neutralize any potential hazards. Also, they were properly disposed of in red bags. This conscientious approach prevented the risk of contamination and the unintentional spread of microorganisms beyond our controlled environment.
AureoBos is highly committed to ethical responsibility, privacy, and the security of individuals involved in our human practices research. Our investigation was developed with respect and in alignment with data protection regulations in Mexico. We created a consent form based on the Federal Law on Protection of Personal Data Held by Private Parties, which governs and manages all the personal data collected and is established in the Political Constitution of the United Mexican States. The consent form underwent a review by our legal team and instructors and was subsequently approved.
Throughout every interview, our primary concern was to maintain the privacy and data confidentiality of the aspects that individuals preferred to keep private. We ensured that they felt protected. To achieve this, before each interview, we presented the consent form to inform them about the personal data being collected, the purpose of the investigation, and the privacy measures being followed, If they agreed the consent form was signed.
1. Public Health Department. (2018). Laboratory Biosafety and Biosecurity Courses. June 2019, de Government of Canada Sitio web: https://training-formation.phac-aspc.gc.ca/?lang=en