OVERVIEW
In order to make our ABC system convenient and efficient to work indoors, we designed a hardware named BioTank to serve as a culture and working container for the entire system.
The device we envision consists of two parts, namely the Main working device and the Auxiliary support device .
Figure 1 : Concept diagram of BioTank
Figure 2 : Picture of BioTank
The Main working device consists of a cube glass cylinder (picture 3) with an opening above and a lid with a cylindrical opening (figure 4).
Figure 3 : D esign drawing of Main working device
Figure 4 : Design drawing of lid
Each of the four inner walls has a indicat ing groove for placing sodium alginate embedded pellets containing E. coli modified to indicate contaminated gases . Indicating groove's exterior walls are fitted with filters that filter light of fixed wavelengths. We selected a filter with a wavelength above 490nm to pass through.(figure 5) The main peak of sfGFP emission light is 507nm, and the main peak of excitation light is 488nm. In this way, under the excitation light, the E. coli with formaldehyde indication function will emit green fluorescence when the formaldehyde exceeds the standard, and through the filter light we can remove the interference of the excitation light and clearly see whether the E. coli emits light.
Figure 5 : Main working device with a light filter
The lid of the Main working device has a cylindrical opening, which can be bound with the common sealing film(picture 6) in the laboratory to prevent the system from being contaminated by external microorganisms, while not affecting the flow of system gases.
Figure 6 : picture of lid with seal film
Auxiliary support device
Because Main working device needs to work together with a lighting lamp and a water pump, the first consideration when designing the bottom display platform is to leave enough space for these two devices
Figure 7 ： skeleton of Auxiliary support device
In order to make this device look more substantial and beautiful, after some consideration, we have decided to refer to the design of the pyramid and add walls and observable internal glass windows. At the same time, in order to facilitate the placement of the device and the wiring of the air pump, we have specifically left a wall space for it to use as a door.
Figure 8 ： C oncept diagram of Auxiliary support device
Figure 9 ： Picture of Auxiliary support device
After the final design is completed, the upper cultivation tank and the lower display platform are independent and separable from each other, but when they are combined, they form our experimental device, which is very beautiful when the algae emit light and the air pump is turned on.