team_logo

Composite Parts

Name Description number link
araC-araBAD-xisF4-PA0717-PA0718-PA0719-PA0720-PA0722-CoaB-PA0724-PA0725--PA0726-aiiA -PA0727 The gene aiiA , encoding quorum quenching enzyme, was strategically integrated within PA0726 with an objective to attenuate biofilm formation, and its regulation is orchestrated by the arabinose operon. The arabinose operon, encompassing the araC gene and araBAD promoter, features AraC as the regulatory fulcrum, deftly orchestrating araBAD expression in sync with arabinose presence. Bba_K4747017

http://parts.igem.org/Part:BBa_K4747017

araC-araBAD-xisF4-PA0717-PA0718-PA0719-PA0720-PA0722-CoaB-PA0724-PA0725-PA0726--ytnP -PA0727 The ytnP gene, encoding a quorum-quenching enzyme, was astutely nestled within PA0726, aiming to mitigate biofilm accretion, with its regulation finely tuned by the arabinose operon. The arabinose operon, encompassing the araC gene and araBAD promoter, positions AraC as the regulatory linchpin, adeptly choreographing araBAD expression in harmony with arabinose availability. Bba_K4747018

http://parts.igem.org/Part:BBa_K4747018

araC-araBAD-xisF4-PA0717-PA0718-PA0719-PA0720-PA0722-CoaB-PA0724-PA0725-PA0726--wspF -PA0727 The wspF gene, a key inhibitor of the c-di-GMP pathway, was adeptly inserted within PA0726 aiming to curb biofilm formation, with its regulation finely tuned by the arabinose operon. This operon, housing the araC gene and araBAD promoter, positions AraC as the regulatory nexus, adroitly coordinating araBAD expression in harmony with arabinose availability. Bba_K4747019

http://parts.igem.org/Part:BBa_K4747019

araC-araBAD-xisF4-PA0717-PA0718-PA0719-PA0720-PA0722-CoaB-PA0724-PA0725-PA0726--yhjH -PA0727 The yhjH gene, encoding the phosphodiesterase (PDE) of the c-di-GMP pathway, was adeptly integrated within PA0726 aiming to mitigate biofilm formation, under the regulatory ambit of the arabinose operon. This operon, housing the araC gene and araBAD promoter, positions AraC as the regulatory pivot, adroitly coordinating araBAD expression in harmony with arabinose availability. Bba_K4747020

http://parts.igem.org/Part:BBa_K4747020


Basic Parts

Name Description number link
araC The araC gene encodes the AraC protein, a transcriptional regulator in bacteria. It controls the arabinose operon, which is involved in the metabolism of the sugar arabinose. The AraC protein's activity is modulated by arabinose, enabling the bacteria to respond to the presence of this sugar in the environment. Bba_K4747000

http://parts.igem.org/Part:BBa_K4747000

araBAD The araBAD gene is part of the arabinose operon in bacteria like E. coli , crucial for the metabolism of arabinose sugar. It encodes enzymes needed to convert arabinose into xylulose-5-phosphate, an intermediate in the pentose phosphate pathway, thus allowing the utilization of arabinose as a carbon source. Bba_K4747001

http://parts.igem.org/Part:BBa_K4747001

xisF4 xisF4 is an excisionase gene found in pf4 filamentous prophage, playing a crucial role in controlling the lysis-lysogeny decision by activating pf4 phage replication via upregulating the phage initiator gene (PA0727)​​. Additionally, it promotes transcription of an operon, which might be involved in the lysis-lysogeny switch, making it a significant factor in Pf prophage excision and replication decisions. Bba_K4747002

http://parts.igem.org/Part:BBa_K4747002

PA0717 PA0717 is potentially located in various cellular compartments including the cytoplasm and membranes Bba_K4747003

http://parts.igem.org/Part:BBa_K4747003

PA0718 PA0718 is related to Pseudomonas aeruginosa PAO1 and is mentioned in the context of cytoplasmic membranes, periplasmic outer membranes, and extracellular pathways. Bba_K4747004

http://parts.igem.org/Part:BBa_K4747004

PA0719 This protein is part of an operon that includes PA0717 and PA0718, and is mentioned in the context of a region encoding various elements associated with Pseudomonas aeruginosa filamentous bacteriophages, which play roles in the pathogenicity of this bacterium, including biofilm formation and suppression. Bba_K4747005

http://parts.igem.org/Part:BBa_K4747005

PA0720 PA0720 is encoding various elements associated with Pseudomonas aeruginosa filamentous bacteriophages and associated with the cytoplasmic membrane and periplasmic outer membrane in Pseudomonas aeruginosa PAO1. Bba_K4747006

http://parts.igem.org/Part:BBa_K4747006

PA0722 PA0722 is associated with protein drug targets, transposon insertions, and has evidence indicating its role as a virulence factor. Bba_K4747007

http://parts.igem.org/Part:BBa_K4747007

CoaB CoaB is associated with the pf4 prophage, which plays a role in the biofilm development of Pseudomonas aeruginosa, contributing to biofilm maturation, cell death, dispersal, and formation of small-colony variants (SCVs) within biofilms. Bba_K4747008

http://parts.igem.org/Part:BBa_K4747008

PA0724 PA0724 is associated with the Pseudomonas aeruginosa PAO1 strain and is situated in the extracellular region. Bba_K4747009

http://parts.igem.org/Part:BBa_K4747009

PA0725 PA0725 is associated with the cytoplasmic membrane in Pseudomonas aeruginosa PAO1, and has been identified through a computational strategy and a laboratory-based PhoA fusion screen aimed at discovering exported proteins of the bacterium. Bba_K4747010

http://parts.igem.org/Part:BBa_K4747010

PA0726 PA0726 plays the role of filamentous phage pf4 in biofilm formation and virulence in P. aeruginosa . Specifically, pf4 is essential for several stages of the biofilm life-cycle and significantly contributes to the virulence of P. aeruginosa in vivo. Bba_K4747011

http://parts.igem.org/Part:BBa_K4747011

PA0727 PA0727 is a Pf replication initiator protein located within the cytoplasmic membrane of P. aeruginosa PAO1. Bba_K4747012

http://parts.igem.org/Part:BBa_K4747012

aiiA The aiiA gene encodes an enzyme that hydrolyzes N-acylhomoserine lactones (AHLs), disrupting quorum sensing in Gram-negative bacteria. This interference with bacterial communication can control bacterial infections and biofilm formation, making aiiA a notable gene in biotechnology and medical research for combating bacterial virulence. Bba_K4747013

http://parts.igem.org/Part:BBa_K4747013

ytnP The ytnP gene encodes a lactonase-homologous protein, and was heterologously expressed in E. coli to study its potential in improving hygiene issues related to biofilm infections caused by Pseudomonas aeruginosa in dental units. Bba_K4747014

http://parts.igem.org/Part:BBa_K4747014

wspF The wspF gene is part of a chemosensory signal-transduction operon in Pseudomonas aeruginosa, , as it interacts with wspF, a signal transduction protein. This gene encodes the regulatory protein for the diguanylate synthase, wspF, and its deletion results in elevated production of certain matrix proteins and cyclic-di-GMP, promoting biofilm formation with significantly more biomass. Bba_K4747015

http://parts.igem.org/Part:BBa_K4747015

yhjH The overexpressed yhjH gene mediates cyclic-di-GMP degradation in Salmonella typhimurium, with the gene encoding a protein containing a cyclic-di-GMP phosphodiesterase EAL domain,influencing biofilm formation and adhesion of bacteria by acting as a second messenger. Bba_K4747016

http://parts.igem.org/Part:BBa_K4747016


footer