In this section, we showcase our tangible contributions to the IGEM community, drawing from our experiences and solutions to the unique challenges we encountered. Our focus centered on Phenylketonuria (PKU), a condition that lacks convenient and effective treatment options. To bridge this gap, we initiated a groundbreaking microbiome therapy project for PKU, offering a promising avenue for future IGEM teams. We designed our project holistically, encompassing in-depth information on PKU, the creation of PKU-treating E. coli strains, and robust biosafety measures.

For future IGEM teams venturing into PKU research, our project serves as a comprehensive reference. We delve into the causes, symptoms, and existing treatment options of PKU, equipping teams with a strong foundation in understanding the condition. This knowledge empowers researchers to navigate the complexities of PKU and craft innovative solutions.

Our pioneering approach involves utilizing synthetic biology to engineer E. coli strains capable of treating PKU. To ensure safe and effective implementation, we provide detailed insights into the design of our microbial chassis and biosafety locks. These precautions serve as a valuable resource for teams working with genetically modified organisms, fostering a culture of biosafety within the IGEM community.

To assess the efficacy of Phenylalanine ammonia-lyase (PAL), a key component in PKU treatment, we developed three comprehensive methods for PAL activity measurement and analysis. These analytical tools offer future IGEM teams a practical means of evaluating PAL's performance in phenylalanine degradation.

Our project contributes tangibly to the IGEM community by presenting a novel approach to PKU treatment and delivering comprehensive resources for teams interested in this field. We aim to inspire and empower future IGEM teams to pursue innovative solutions for PKU and related challenges in synthetic biology.

In concluding our IGEM journey, we extend our gratitude to the IGEM community for its unwavering support and the collaborative spirit that defines our shared mission. We anticipate witnessing the remarkable achievements of future IGEM teams as they build upon our contributions and continue to address pressing global issues through synthetic biology.