Background:

In the dynamic realm of the International Genetically Engineered Machine Competition (iGEM), collaboration stands as a cornerstone for propelling scientific endeavors. It's within this spirit of synergy that our BFSU-ICUnited team, dedicated to Bio Conditioner innovation, found common ground with the Squirrel-CHN team, pioneers in curing Phenylketonuria (PKU), centered around the critical enzyme, tyrosinase. Recognizing our shared need for this enzymatic powerhouse, we embarked on a collaborative journey aimed at maximizing resources and efficiency.

Our collaborative efforts took root in face-to-face discussions, followed by the establishment of a WeChat group and online meetings, serving as virtual crucibles of ideas.

Together, we embarked on a dual-pronged approach to enzyme synthesis, focusing on two vital components:

1. Shared Laboratory Equipment and Resources:

   - Mutual Chemicals: Our collaboration included the exchange of essential chemicals, including tyrosine, p-chlorophenylacetic acid (PCPA, an inhibitor of tyrosinase), TMB substrate (employed for measuring tyrosinase activity), as well as common buffers and enzyme inhibitors.

   - Equipment Synergy: Beyond chemicals, we pooled our resources further, generously sharing laboratory equipment such as centrifuges and thermostatic water baths. This collaborative equipment sharing significantly facilitated our respective experimental processes.

2. Ongoing Exchanges and Mutual Learning:

Our collaboration extended beyond resource sharing. It became a platform for addressing challenges that both teams encountered:

   - Stability Conundrums: The Bio Conditioner team grappled with the issue of tyrosinase stability under specific conditions, while the Cure PKU team faced the challenge of achieving optimal enzyme yield in mass production.

In Conclusion:

Our collaborative efforts have yielded not only substantial progress within our individual projects but also the strengthening of bonds and trust between our teams. This shared journey has not only enhanced our competitiveness in the iGEM competition but has also paved the way for greater innovations in scientific research. As we continue our partnership, we remain steadfast in our belief that our shared endeavors will lead to groundbreaking achievements and advancements in the world of genetic engineering. Together, we stand poised to make a lasting impact.