| pET_fwd | TAATACGACTCACTATAGGG |
| pET_rev | GCTAGTTATTGCTCAGCGGT |
| sfGFP_fwd | ggctacggtctcaaatgCGTAAAGGCGAAGAGCTGTTCACTG |
| sfGFP_rev | ggctacggtctccaagctcaTTTGTACAGTTCATCCATACCATGCGTGA |
| pET28-noLacI-BsaI_rev | ggctacggtctcacattAGAGGGGAATTGTTATCCGC |
| pET28-BsaI_fwd | ggctacggtctcacattAGAGGGGAATTGTTATCCGC |
| pET28-BsaI_rev | ggctacggtctcacattAGAGGGGAATTGTTATCCGC |
| pET28+LacI+BsaI-T7_rev | ggctacggtctcacattAGAGGGGAATTGTTATCCGC |
| Anderson+vsfGFP+BsaI_rev | ggctacggtctcaaagcTATAAACGCAGAAAGGCC |
| pET28 Anderson Colony PCR_fwd | CATACCCACGCCGAAACA |
| Part 1 (Promoter & BmoR) FWD | TGTCTCGGTCTCCaatgTTGACGGCTAGCTCAGTC |
| Part 1 (Promoter & BmoR) REV | TGTCTCGGTCTCCAGTACCGATGCGAGACTG |
| Part 2 (terminator + UAS +pBMO + RBS) FWD | TGTCTCGGTCTCGTACTTAAGCTTAACCAGGCATCAAAT |
| Part 2 (terminator + UAS +pBMO + RBS) REV | TGTCTCGGTCTCGTGAGTATTTCTCCTCTTTCTCTAG |
| Part 3 (vsfGFP terminator) FWD | TGTCTCGGTCTCGCTCATATGTCTAAGGGTGAAGAATTGT |
| Part 3 (vsfGFP terminator) REV | TGTCTCGGTCTCGaagcTATAAACGCAGAAAGGCCC |
| pJUMP28-1A Fwd | ggctacggtctctgctttgactgagttgcacgctg |
| pJUMP28-1A Rev | ggctacggtctctcattgtatatctccttcttaaagttaaaca |
| pET28a-BsaI Fwd | ggctacggtctcagcttTGAGATCCGGCTGCTAAC |
| pET28a-BsaI Rev | ggctacggtctcacattGTATATCTCCTTCTTAAAGTTAAACAAAATT |
| Part 1 primer attempt 2 Fwd | TTGACGGCTAGCTCAGT |
| Part 1 primer attempt 2 Rev | TTAAGTACCGATGCGAGAC |
| pBMO Fwd | GGTCTCccacagatagtaggtgctgcggc |
| vsfGFP Fwd | GGCTACGGTCTCAAATGTCTAAGGGTGAAGAATTGTTCACAGGAGTTG |
| vsfGFP-0 Fwd | GGCTACGGTCTCAAATGATGTCTAAGGGTGAAGAATTGTTCACAGGAG |
| vsfGFP-0 Rev | GGCTACGGTCTCCAAGCTCAAGAAACAGTGACTTGCGTTCCTTGG |
| PS1 (Differentiate construct and sfGFP) | GGCTACGGTCTCCAAGCTCAAGAAACAGTGACTTGCGTTCCTTGG |
| PS2 (Differentiate construct and sfGFP) | GCGGCAACCGAGCGTTC |
| pET28 BsaI Rev (right overhang for TIIS) | ggctacggtctcgctccATTTCGCGGGATCGAGATC |
| pET28 BsaI Fwd (right overhang for TIIS) | ggctacggtctcccgctTGAGATCCGGCTGCTAAC |
| J23100+vsfGFP Fwd (right overhang TIIS) | ggctacggtctcaggagTTGACGGCTAGCTCAGTC |
| J23100+vsfGFP Rev (right overhang TIIS) | ggctacggtctctagcgTATAAACGCAGAAAGGCC |
| J23100+vsfGFP+BsaI Fwd | ggctacggtctctaatgTTGACGGCTAGCTCAGTC |
| J23104+vsfGFP+BsaI Fwd | ggctacggtctcgaatgTTTACGGCTAGCTCAGTC |
| J23106+vsfGFP+BsaI Fwd | ggctacggtctctaatgTTTACGGCTAGCTCAGTC |
| J23110+vsfGFP+BsaI Fwd | ggctacggtctcaaatgTTTACGGCTAGCTCAGTC |
| J23114+vsfGFP+BsaI Fwd | ggctacggtctccaatgTTTATGGCTAGCTCAGTC |
| J23119+vsfGFP+BsaI Fwd | ggctacggtctcgaatgTTGACAGCTAGCTCAGTC |
| pET28a+Lacl+BsaI-T7 Rev | ggctacggtctcacattATTTCGCGGGATCGAGAT |
| 77 gp104 Fwd | TCCTTCGGTCTCCAATGatggtaacgaaagaatttttaaaaac |
| 77 gp104 Rev | TCCTTCGGTCTCCAAGCttaatattcaacaatcgctggg |
| BmoR without RS for stuff we don’t like Fwd | TGACGTGGTCTCGAATGATGTCCAAAATGCAGGAGT |
| BmoR without RS for stuff we don’t like Rev | TGACGTGGTCTCGAAGCTTAAGTACCGATGCGAGAC |
| Full pBMO + UAS Fwd | CCGAAAGGTCTCGggagccacagatagtaggtgctg |
| Full pBMO + UAS Rev | CCGAAAGGTCTCGAGTActctaacgctttcagcaag |
| G1 gp240 FWD | CGAGATGGTCTCGAATGatggtaatcccaagtatcaagg |
| G1 gp240 REV | CGAGATGGTCTCGAAGCttactcaccataacgctcc |
| G1 gp240_Fwd | ggctacggtctcgaatgATGGTAATCCCAAGTATCAAG |
| G1 gp240_Rev | ggctacggtctcgaagcTTACTCACCATAACGCTC |
| Optimised vsGFP (for the biosensors) Fwd | CCGAAAGGTCTCCAATGCATatgTCTAAGGGTGAAGAAC |
| Optimised vsGFP (for the biosensors) Rev | CCGAAAGGTCTCCAAGCCTCGAGtcaGCTAACAGT |
| pET28a-noLacI-BsaI Rev | ggctacggtctcacattGCGCAACGCAATTAATGTAAG |
| T4 Alc Fwd | ggctacggtctcaaatgATGGATCTGCAGCTTATC |
| T4 Alc Rev | ggctacggtctcgaagcTTACATGCAGAGAGTTTTG |
| T4 AsiA Fwd | ggctacggtctcaaatgATGAATAAAAACATCGACACAG |
| T4 AsiA Rev | ggctacggtctctaagcTTATTTGTTCGTATACATCTCTAG |
| T7 gp0.7 Fwd | ggctacggtctcgaatgATGAAAGAGATTGACCGTG |
| T7 gp0.7 Rev | ggctacggtctccaagcTTAGCCCATTAACATTGC |
| T7 gp2 Fwd | ggctacggtctctaatgATGTCAAACGTAAACACG |
| T7 gp2 Rev | ggctacggtctcgaagcTTACTTTGGTGCTACACAAG |
| T7 gp79 Fwd | ggctacggtctcaaatgATGGATATGTTTTCACTCG |
| T7 gp79 Rev | ggctacggtctctaagcTTACAGATAGGTAACGCC |
| T7 gp104 Fwd | ggctacggtctcaaatgATGGTAACGAAAGAATTTTTAAAAAC |
| T7 gp104 Rev | ggctacggtctccaagcTTAATATTCAACAATCGCTGG |