Overview
The OUC-Haide 2023 project is dedicated to utilizing synthetic biology and the non-natural coenzyme NCD to combat formaldehyde pollution in the air. In pursuit of this goal, we have introduced six new basic parts (Table 1), which can be broadly categorized into two sections.
The first section (FtNadE, CtCTPs, Ncds-2) pertains to the synthesis of the non-natural coenzyme NCD. This section comprises enzymes that facilitate the production of NMN and CTP, along with enzymes that enable the creation of NCD. NMN and CTP serve as precursor substances essential for the generation of NCD.
The second section (FalDH, FDH, ME) focuses on the formaldehyde metabolism pathway. This segment includes modified formaldehyde dehydrogenase, formate dehydrogenase, and malic enzyme. These enzymes have undergone modifications within their active sites to adapt to NCD, ensuring that they can metabolize formaldehyde without disrupting the natural coenzyme NAD within engineered bacteria. The presence of malic enzyme plays a crucial role in catalyzing NCDH into NCD, thereby maintaining the equilibrium of NCD within engineered bacteria.
New Parts
| Part Name | Part Registry ID | Type |
|---|---|---|
| Ncds-2 | BBa_K4596001 | Coding |
| CtCTPs | BBa_K4596002 | Coding |
| FtNadE | BBa_K4596003 | Coding |
| FalDH | BBa_K4596004 | Coding |
| FDH | BBa_K4596005 | Coding |
| ME | BBa_K4596006 | Coding |