Notebook

December 5, 2022

The NPU-CHINA team was established.

December 25, 2022

The first topic selection meeting was held, with an initial feasibility study of the proposals being conducted.

February 10, 2023

The second topic selection meeting took place, finalizing the project for this time.

March 14, 2023

Research and collection of data related to the project commenced.

April 13, 2023

Communication and exchange with the iGEM team from Northwest University.

May 7, 2023

Discussion on launching iGEM promotional activities within the campus.

May 9, 2023

The designed plasmid was handed over to Tsingke Biotechnology for synthesis.

May 12, 2023

The first induction expression was initiated.

May 31, 2023

Multiple attempts at inducing expression failed, prompting further research into genes from other sources.

June 13, 2023

It was decided to use a new gene from the Paraffin-like Bacillus to construct the plasmid.

June 16, 2023

Exploration of conditions for the induction expression experiment began.

August 12, 2023

Purified expressed protein was obtained, marking the start of protein modification efforts.

October 4, 2023

Enzyme activity was measured.