Attributions

Attribution Form

Internal Contributors

Team Members Contributions

Name	Role	General Tasks	Specific Tasks
Fien Eickmans	Student	Analysis Conceptualization Background Research Investigation Public Engagement Writing	Analysis - She performed analysis of the DNA sequencing results and the Wilbur-Anderson assay data. Investigation - As co-head of the wet lab, she lead and organised the experiments relevant to our project. Conceptualization - She designed all parts in SnapGene and played an important role in designing lab protocols. Background Research - Conducted research for the project development and the curation of the research plan, especially focusing on the DNA origami module. Public Engagement - Conducted guest lessons for middle school and post-high school students and represented the 2023 iGEM team at the MSP student fair. Writing - Contributed to the Engineering, Education wiki pages and the parts registry.
Floor Vervuren	Student	Analysis Investigation Background Research Public Engagement Writing	Analysis - Performed analysis of the DNA sequencing results and the WA assay data. Analysed TEM data from DNA origami. Investigation - Performed wet lab experiments and assisted with testing the modules in and outside the laboratory. Background Research - Conducted research for the project development and the curation of the research plan. Public Engagement - Conducted a guest lesson for post-high school students and represented the 2023 iGEM team at the MSP student fair. Was partly responsible for keeping our social media updated. Writing - Contributed to the Engineering, Education and Human Practices wiki pages. Played in major in documenting our parts on the registry. Was responsible for writing and publishing a series of blogs about the project.
Ramón Reszat	Student	Background Research Investigation Fundraising Software	Software - Developed enzyme kinetics model for our bacteria and running molecular dynamics simulations of the DNA origami design, organized the team’s software repository. Writing - Contributed to the Modeling wiki page, created the teams external presentation slides. Background research - Performed literature review on applications of DNA templated nanomaterials. Fundraising - Reached out to potential sponsors for research material and expertise, email communication with external institutes.
Chris Damour	Student	Analysis Conceptualization Background Research Data Curation Project Administration Software Wiki Coding Visualization Writing	Analysis - Analysed TEM data from DNA origami Conceptualization - Established the idea of nucleation sites with DNA origami and the design of the lattice. Background Research - Lead the research on DNA origami including the fabrication of single stranded DNA with the dual plasmid system and design of the lattice. Data Curation - Curated the data for DNA origami including model prediction and TEM imaging Project Administration - Developed the project management software and guided team members with non-lab related tasks. Software - Established the DNA origami models and developed a script to generate the sticky ends. Wiki Coding - Coded the technical aspect of the wiki, including writing a script that synchronised our project management software and our wiki. Visualization - Visualized the DNA origami alongside producing animations for both video deliverables. Writing - Contributed to writing for the wiki.
Natalia Bermejo Gago	Student	Public Engagement Fundraising Writing	Public Engagement - Reached out to SRAL and raise awareness for our project among local art students. Background research - Conducted background research during the project development related mostly to the origami module. Fundraising - Contacted various biotech and regional companies as well as set up the various crownfunding campaigns. Writing: participated in the wiki writing specially related to human practices and helped in the blog posts.
Natalia Bartos	Student	Investigation Notebook Public Engagement Writing Fundraising Other	Notebook - Helped keeping track of the details regarding lab experiments. Investigation - assisted in the wet lab throughout the project. Public Engagement - Contacted teams regarding collaboration Fundraising – Assisted the team in sending emails to potential sponsors. Writing – Contributed to wiki content, wrote scripts for educational videos, also assisted with proofreading and editing text in various parts of the project Other - Currently working on an ongoing collaborative educational project about synthetic biology.
Alicia Del Prado Rebordinos	Student	Investigation Project Administration Fundraising Public Engagement Entrepreneurship Visualization Writing	Investigation - Assisted with some experiments in the lab. Project Administration - Managed the calendar, organised meetings and coordinated different team members. Helped solve internal issues. Fundraising - Took care of finding and writing applications for available grants. Participated in establishing the crowdfunding avenues. Public Engagement - Interviewed an expert from the SRAL institute, edited and uploaded it to YouTube. Represented the 2023 iGEM team at the MSP student fair. Entrepreneurship - Came up with a contacting strategy and provided the organisation space for managing the funding. Found and contacted potential sponsors. Visualization - Made the promotional video and the presentation video. Writing - Reviewed and wrote content on grant applications and project presentations.
Mara Taracena Wulff	Student	Analysis Conceptualization Background Research Investigation	Investigation - As co-head of the wet lab, she lead and organised most of the experiments relevant to our project. Conceptualisation - Defined our two bacterial systems along with Chris and Fien. Developed theoretical aspects of the mineralisation (SIMD) system. Curated protocols for the activity and mineralisation assay, DNA origami folding experiments, alginate bead entrapment, protein extraction, and thermogravimetric analysis. Analysis - interpreted and analysed all experimental results for the SIMD module. Analysed DNA origami folding experimental data. Compared data with existing literature, catalogued experimental designs. Background Research - Performed the literature review for the project and contributed to the curation of the research plan. Co-lead research activities for both modules. Writing - contributed to the Engineering, Composite Part, and Project Description wiki pages.
Matei Marusciac	Student	Investigation Analysis Background Research Public Engagement Fundraising	Investigation - helped and performed some of the biology based experiments and conducted the chemistry based experiments. Analysis - Performed interpretation of collected ATR-IR data of the produced compounds. Background Research - Research done on analytical techniques, measurements and chemical-related results Public Engagement- Held lecture to educate peers and raising awareness for the project through both social media and social interactions, participate in writing grants and template emails. Involved in the promotional video. Fundraising - Found potential sponsors and contacted already existing and new leads, as well as actively participating in holding meets that materialised into successful funding operations.
Isabella Walbeck	Student	Investigation Notebook Public Engagement Visualization Writing Other	Investigation - Helped with wet lab throughout the project. Notebook - Tracked experiments in the notebook and also designed the cover page. Public engagement - Was one of the speakers at a pitching event and at an educational lecture. Worked on funding by writing grants and contacting possible funding avenues. Visualization - Designed our team logo, established our brand identity and did part of the animations on the ABCDna video series. Created some of the figures used in presentations and posts in social media. Writing - Helped with writing scripts for the ABCDna videos as well as grant applications.
Iga Kosarczyn	Student	Investigation Background Research Notebook Public Engagement Visualisation Writing	Investigation - Performed the transformation of our constructed plasmid into E.coli, assisted in the testing of the modules in lab. Background Research - Assisted with the research for the project’s development. Notebook - Created a “wet lab” notion page in order to keep a record of laboratory activities. Public Engagement - Participated in the SRAL interview. Visualisation - Assisted Isabella with the team logo design and brand identity, did part of the of the animations on the educational ABCDna video series. Designed/drew posts for the social media page. Writing - Wrote parts of the scripts for educational videos as well as contributed in the writing of wiki pages.
Erik Steen Redeker	PI	Conceptualization Supervision Background Research Safety	Conceptualization - Guided the team throughout the research, including suggesting DNA origami. Supervision - Supervised all of the work our team did in the laboratory. Background Research - Reading scientific research articles, regulations/guidelines, and other documents. Safety - Performing activities to ensure compliance with the safety guidelines or requirements of iGEM, Maastricht University, and the Dutch government.
David Cortens	PI	Background Research Supervision	Background Research - Reading peer-reviewed scientific research articles, regulations/guidelines, and other documents. Supervision - Supervised all of the work our team did in the laboratory.
Kathia Jimenez-Monroy	Instructor	Investigation Supervision	Investigation - Assisted with using laboratory equipement while ensuring the safety guidelines. Supervision - Supervised all of the work our team did in the laboratory while providing assistance to our team members whenever needed.

External Contributors

Name	Institution Type	Relationship to Team	General Tasks	Specific Tasks
GenScript	Industry	Sponsor	Investigation	Provided us with a plasmid preparation service
Maastricht University	Academic	Sponsor	Fundraising	Provided us with financial support
Macrogen	Industry	Sponsor	Investigation	Provided us with sequencing
Integrated DNA Technologies (IDT)	Industry	Sponsor	Investigation	Provided us with G-Blocks for SazCa module and synthesised all of our staples
Ramiro Marroquin Garcia	Academic	External Advisor	Conceptualization	Researcher PhD Candidate at Maastricht University, provided us with an idea that would help us in achieving proof of concept - testing of our SazCa enzyme by putting bacteria in alginate gel
Massimiliano Simons	Academic	External advisor	Conceptualization	Assistant Professor in Philosophy of Technology at Maastricht university offered us guidance and insight into the ethics of both our project and synthetic biology during an interview
Kate Seymour	Other	External Advisor	Conceptualization	Head of education and training / Senior paintings conservator at SRAL She offered us insight into paintings conservation techniques, and how our project could be implemented through an interview.
Maximilian Nicolas Honemann	Academic	External Advisor	Conceptualization	M.Sc Biochemistry, Senior Scientist at capsitec GmbH He provided us with valuable insight on the production of ssDNA.
Kevin Knoops	Academic	External Advisor	Investigation	Microscopy Specialist at Maastricht University, who provided us with assistance and availability to use TEM microscope in order for us to visualise samples.
Hans Duimel	Academic	External Advisor	Investigation	Education-/Research Officer who perform our TEM sample preparation and TEM setup. Guided us through using the microscope.
Claudia Vickers	Academic	External Advisor	Conceptualization	During an interview, she provided us with the insight into synthetic biology and its legal aspects
Marlowe Ural	Academic	External Advisor	Investigation Analysis	MSP student, Investigation: helped us perform sonication, IR preparation and offered assistance in different chemical techniques, such as vacuum filtration and other separation methods. Analysis: helped the team in IR data analysis

Project Timeline

Project Activity	Start Date	End Date	Total Time
Recruit Team Members	09/12/2022	11/02/2023	9
Development of Project Plan	7/03/2023	20/06/2023	15
Wet Laboratory Activities	11/07/2023	06/10/2023	13
Dry Laboratory Activities	25/06/2023	06/10/2023	16
Building the Team Wiki	26/06/2023	12/10/2023	16