Parts


Utilized and characterized existing parts


Part name Part number Contribution
pTDH3 BBa_K124002 Gene expression proved
tTDH1 BBa_K2753052 Gene expression proved
pPGK1 BBa_K4012003 Gene expression proved
tPGK1 BBa_K3126012 Gene expression proved
pTEF2 BBa_K165037 Gene expression proved
tSSA1 BBa_K4012020 Gene expression proved
tHMG1 BBa_K4121019 Used to overexpress HMGR to help construct a terpenoids producing strain. We have proved that the expression of tHMG1 and IDI1 in yeast can increase the metabolite flux of MVA pathway.
IDI1 BBa_K4121025 Used to overexpress IDI1 to help construct a terpenoids producing strain. We have proved that the expression of tHMG1 and IDI1 in yeast can increase the metabolite flux of MVA pathway.
ERG20_F96W BBa_K4274002 Used to overexpress ERG20 and collaborate with ClSS in yeast. We have proved that its expression together with ClSS can contribute to the synthesis of santalene in yeast. Based on the overexpression of ERG20, the heterologous expression of CYP736A167 and SaCPR2 can contribute to the synthesis of santalol in yeast.





New basic parts


Part name Part number Sequence correct? Does it function? Group favorite?
BmeTC BBa_K4805000 Yes No No
BmeTC_Y167A,D373C BBa_K4805001 Yes No No
BmeTC-FL-BmeTC Y167A,D373C BBa_K4805002 Yes No No
ClSS BBa_K4805003 Yes Yes Yes
ClSS_S532A/F441V BBa_K4805004 Yes Yes No
CYP736A167 BBa_K4805005 Yes Yes Yes
SaCPR2 BBa_K4805006 Yes Yes Yes




Composite Parts


Part name Part number Composite of: Sequence correct? Does it function? Group favorite?
pTDH3-tHMG1-tTDH1-pPGK1-IDI1-tPGK1 BBa_K4805007 BBa_K124002+BBa_K4121019+BBa_K2753052+BBa_K4012003+BBa_K4121025+BBa_K3126012 Yes Yes Yes
pTDH3-BmeTC_Y167A,D373C-tTDH1-pTEF2-ERG20_F96W-tSSA1-pPGK1-BmeTC-tPGK1 BBa_K4805008 BBa_K124002+BBa_K4805001+BBa_K2753052+BBa_K165037+BBa_K4274002+BBa_K4012020+BBa_K4012003+BBa_K4805000+BBa_K3126012 Yes No No
pTDH3-BmeTC-FL-BmeTC Y167A,D373C-tTDH1 BBa_K480509 BBa_K124002+BBa_K4805002+BBa_K2753052 Yes No No
pTEF2-ERG20_F96W-tSSA1-pPGK1-ClSS-tPGK1 BBa_K4805010 BBa_K165037+BBa_K4274002+BBa_K4012020+BBa_K4012003+BBa_K4805003+BBa_K3126012 Yes Yes Yes
pTEF2-ERG20_F96W-tSSA1-pPGK1-ClSS_ S532A,F441V-tPGK1 BBa_K4805011 BBa_K165037+BBa_K4274002+BBa_K4012020+BBa_K4012003+BBa_K4805004+BBa_K3126012 Yes Yes No
pPGK1-SaCPR2-tPGK1-pTDH3-CYP736A167-tTDH1 BBa_K4805012 BBa_K124002+BBa_K4805006+BBa_K2753052+BBa_K4012003+BBa_K4805005+BBa_K3126012 Yes Yes Yes