To validate our protocol, we designed the following synthetic biological parts. We show the basic components and combinations of parts we designed below, and register them on the official part library of the igem to give other igemers a better display.
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K4609050 | Coding | A protein that binds to AHL to induce the Lux pR promoter | 2063 bp |
| BBa_K4609051 | Coding | A bacteriostatic protein | 869 bp |
| BBa_K4609052 | Coding | A red fluorescence | 810 bp |
| BBa_K4609053 | Coding | A cytotoxin | 452 bp |
| BBa_K4609054 | Coding | Thermosensitive promoter controlled FLP recombinant enzyme | 2052 bp |
| BBa_K4609055 | Coding | A cellular antitoxin | 447 bp |
| BBa_K4609056 | Coding | A heat shock protein that increases intracellular lactose concentration and induces protein expression | 640 bp |
| BBa_K4609057 | Tag | A protein tag | 33 bp |
| BBa_K4609058 | RBS | ribosome bind site | 23 bp |
| BBa_K4609059 | Coding | the Lac Operon | 25 bp |
| BBa_K4609060 | Coding | Arabinose promoter | 876 bp |
| BBa_K4609061 | T7 | A T7 promoter | 19 bp |
| BBa_K4609062 | T7 | A T7 terminator | 48 bp |
| BBa_K4609063 | T7 | A T7 promoter | 28 bp |
| BBa_K4609064 | Coding | A promoter | 55 bp |
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K4609070 | Composite | The DMBT1 expression system can express DMBT1 protein and exert antibacterial effect | 2181 bp |
| BBa_K4609071 | Composite | The self-induction system expresses HSP27 protein and induces the expression of multiple proteins | 755 bp |
| BBa_K4609072 | Composite | Quorum sensing system plays a role in controlling the density of E. coli. | 1801 bp |
| BBa_K4609073 | Composite | Toxin expression system, expressing HicA antitoxin, used to control Escherichia coli dormancy | 1801 bp |
| BBa_K4609074 | Composite | Toxin expression system, expressing HicA antitoxin, used to control Escherichia coli dormancy | 1409 bp |
| BBa_K4609013 | Composite | The antitoxin expression system expresses the HicB antitoxin and functions together with the HicA toxin | 2570 bp |