Through our dedication and hard work, our team has strived to meet the highest standards of excellence and uphold the values of the iGEM competition. On this page, you will find a summary of the work we have accomplished to fulfill the various criteria for medals, special awards, and components.
Competition Deliverables
(Wiki, Project Promotion Video,Presentation Video, Judging Form): we have handed over all Competition Deliverables throughout the iGEM season.
You can find our promotional video on our homepage or on the iGEM website. Communication plays a pivotal role in our project, and as such, we have invested a lot of efforts in our promotional video. We have ensured that it reaches a wide audience by offering it in multiple languages (French, English, Spanish, German, Italian, and Arabic) and providing numerous subtitles (French, English, Spanish, German, Italian, Polish, and Arabic).
Just like our promotional video, our presentation video holds significant importance in relation to our topic. We are committed to effectively communicating about our subject and its context, a crucial skill in raising awareness and educating others about antibiotic resistance. Our video has been produced in English to ensure accessibility to a broad audience, and we have included subtitles in various languages for wider comprehension.
We filled out the attribution form with utmost diligence, you can find it on the Attributions page. Regarding our team members, we have provided detailed information about the teams (division of the team into various groups), individual responsibilities, and specific contributions to the project. We have also meticulously listed all the organizations that have contributed to our project, whether through financial support, scientific collaboration, or other means. Additionally, we included an acknowledgment section to express our gratitude to all those who have contributed to our project throughout our journey.
You can find the Description page on our wiki. First and foremost, we provide an explanation of the context surrounding antibiotic resistance, including its history and its past and future impacts. We then introduce our project: SuperBugBuster. We not only discuss the contributions made by software and human practices but also delve into our two biological approaches: CRISPR and BacProtac. Furthermore, we offer a detailed insight into the future vision of our tool and our motivations for its development. We have made every effort to comprehensively describe our project, both in its entirety and in its finer details.
You can find the Contribution page on our wiki, where we have outlined the various ways our project has contributed to the iGEM community. These contributions are diverse and extensive. We have created tutorials on the use of software tools employed in designing our BacProtacs. Additionally, we've detailed the creation process of our antibiotic-themed Werewolf game, our awareness campaign, social media posts, and collaborations. Our contributions also extend to the biological realm, including the innovation of BacProtac, the development of new composite parts, and our CRISPR system coupled with a mutagenesis module, which can be applied in numerous projects. We have made substantial efforts to enhance the iGEM community through both informational resources and biological innovations.
We have drafted and completed the Engineering page on our Wiki. Our project has undergone multiple iterations of the engineering cycle to achieve our primary goal: combating antibiotic resistance. This cycle was meticulously followed and documented in our Wiki for various components of our project, including all biological aspects, human practices (including education), modeling, and software development.
A thoughtful consideration of the engineering cycle has been pivotal throughout our project, enabling us to align ourselves with the excellence criteria set by iGEM. It has guided our efforts in striving for excellence and addressing the challenges posed by antibiotic resistance.
You can find the Human Practices page on our wiki. Human practices have played a pivotal role throughout our project. We have compiled a comprehensive bibliography and organized it using a methodological tool, which you can access on the page. This process helped us formulate our initial inquiries regarding our tool and served as the foundation for our Integrated Human Practices work.
We have created a model and a computer simulation based on our tool. The code is open-source and user-friendly with tutorials. You can find the code in our gitlab and our model on the Model page of our wiki. We created a model to predict our tool CRISPR dCas9 test results and allow lab members to set initial conditions. To take this further and in an iGEM values-based approach, we have created a second model that builds on the first and provides much more visual results. This approach enables everyone to understand how our model works. Moreover, we've also created a tutorial for future iGEM teams to help them get started with their modeling more easily. In addition, we've also done some modeling on the BacProtac section involving docking to determine the structures of the various elements making up our BacProtac. You can find all our docking work on the Docking page of our wiki.
Our project has been shaped by our human practices research from its inception to its completion. We tackled two highly complex facets of the biotechnology world: therapeutics and antibiotic resistance. It was crucial for us to engage with numerous experts to gather their insights and advice in order to develop a tool that is sustainable, safe, and beneficial for the world. We dedicated considerable effort to these aspects, and our tool reflects this commitment. You can find our Integrated Human Practices page on our wiki.
We have developed a software for designing guide RNAs for our experiments using CRISPR technology. This software is open-source and user-friendly, and you can access it on the Software page of our wiki. It played a pivotal role in the design of our experiments, and we also designed it with the intention that it can be utilized by anyone needing to create guide RNAs, regardless of the experiment's context. It will also be instrumental in the future of our tool, enabling us to redesign our plasmid for various resistance types.
Our team decided to present our project SuperBugBuster, in the Therapeutic Igem village. Indeed, the choice of it was clear. Our tool and our entire project are based on tackling the global challenge of antibiotic resistance. To do so, we aim to remove antibiotic resistance from bacteria. We are treating antibiotic resistance at its core, and because of that, this village was the perfect fit for us.
