Section 1 : Introduction

Biology is different from other engineering fields due to a lack of modular nature of the structure and function, which means the expression of gene will change when transferred from one species to another. What synthetic biology is dedicated to is the modularization and standardization of functional gene, to make the expression of it predictable in different chassis organism. Our project has registered 19 basic parts and 10 composite parts, all qualified for standardized requirement of RFC[10] or RFC[1000]. We have also tested these parts. We hope that everyone can utilize our parts based on our research through registry and that the information for parts can further be completed so that more people can make use of them.

Section 2 : Basic parts

Section 3 : Improvement

In this year, we tried to increase our target products, lactate and electricity, by modulating the total amount of intracellular NAD(H/+). In cyanobacteria we tried to introduce OmcS, an electron transfer protein to achieve an increase in NADH content, which in turn provides more cofactors for lactate dehydrogenase to catalyze more lactate. In Shewanella oneidensis MR-1, we attempted to enhance NADH synthesis by adding new synthetic pathways and increasing the activity of enzymes in the pathway, including nadE, a previously registered part. We performed codon optimization for Shewanella oneidensis MR-1 and further analyzed and predicted the protein structure to provide a reliable basis for experimental research and development, and registered it as a new part BBa_K4595015.

Finally, we successfully constructed four major plasmids, successfully verified the expression of relevant genes in some of them, and detected the enhancement of lactate generated by cyanobacteria with the power production of Shewanella oneidensis MR-1.

Section 4 : Composite parts