| Parts | Description |
|---|---|
| BBa_K4757000 | Fluorescent protein mKate2 |
| BBa_K4757001 | Peptide degradation tag taken.(Halvorsen et al., 2022, BioRxiv) |
| BBa_K4757002 | De-novo synthetic ribosomal binding site designed with the Salis-lab RBS calculator. The calculation was based on the LCC mRNA (BBa_K4757016) to maximize expression in P. fluorescens. |
| BBa_K4757003 | De-novo synthetic ribosomal binding site designed with the Salis-lab RBS calculator. The calculation was based on the mKate2 mRNA (BBa_K4757000) to maximize expression in P. fluorescens. |
| BBa_K4757004 | De-novo synthetic ribosomal binding site designed with the Salis-lab RBS calculator. The calculation was based on the AlkB mRNA (BBa_M36710) to maximize expression in P. fluorescens. |
| BBa_K4757005 | De-novo synthetic ribosomal binding site designed with the Salis-lab RBS calculator. The calculation was based on the SPpstu-FAST-PETase mRNA (BBA_K4757015) to maximize expression in P. fluorescens. |
| BBa_K4757008 | Ps1/Ps2 promoter system controlling XylS expression natively found in Pseudomonas species The promoter consists of two parts (Ps1/Ps2). Ps2 is a σ70-dependent, constitutively active promoter with low expression strength. Ps1 is a σ50-dependent regulative promoter with strong expression strength, it is activated by the XylR transcription factor and through self-induction by XylS |
| BBa_K4757011 | Engineered XylS transcription factor with two point mutations (K38R, L224Q) to change substrate recognition towards terepthalic acid. (Li et al., 2022, ACS Synth. Biol.) |
| BBa_K4757013 | Engineered AlkS transcription factor with a single point mutation (V760E) to shift alkane recognition length up to n-heptadecane. (Chen et al., 2023, ACS Synth. Biol.) With our expremiments we could only show recognition with n-dodecane |
| BBa_K4757015 | Engineered FAST-PETase for more efficient PET degradation. (Lu et al., 2022, Nature) |
| BBa_K4757018 | P.stuzeri secretion tag functional in P. fluorescens |
| BBa_K4757021 | sRNA seed region homologous to the RBS BBa_J61100 |
| BBa_K4757022 | sRNA seed region homologous to the RBS BBa_J61101 |
| BBa_K4757023 | sRNA seed region homologous to the RBS BBa_K4757003 |
| BBa_K4757024 | sRNA seed region partially targeting the RBS (BBa_J61100) and the mRNA of mKate2 (BBa_K4757000) |
| BBa_K4757025 | sRNA seed region partially targeting the RBS (BBa_J61101) and the mRNA of mKate2 (BBa_K4757000) | BBa_K4757026 | sRNA seed region targeting the RBS (BBa_K4757003) and the mRNA of mKate2 (BBa_K4757000) |
| BBa_K4757027 | sRNA seed region targeting the mRNA of mKate2 (BBA_K4757000) |
| BBa_K4757028 | sRNA scaffold MicC (Na et al., 2023, Nature) |
| BBa_K4757029 | sRNA scaffold SgrS. (Noh et al., 2019, ACS Synth. Biol.) |
| BBa_K4757030 | sRNA scaffold SgrS-S CUUU 6 nts stem (SgrSmt). (Noh et al., 2019, ACS Synth. Biol.) |
| BBa_K4757058 | Bi-directional terminator. (Lammens et al., 2023, ACS Synth. Biol.) |
| BBa_K4757110 | Pm promoter activated by XylS binding. |
| Parts | Description |
|---|---|
| BBa_K4757041 | sRNA consisting of a seed region (BBa_K4757025) targeting the RBS (BBa_J61101) with the SgrS scaffold (BBa_K4757029). |
| BBa_K4757059 | Composite part of the XylS-K38R-L224Q transcription factor controlled by the Ps1/Ps2 promoter system |
| BBa_K4757006 | Composite part of the XylS-K38R-L224Q transcription factor controlled by the pEM7 promoter system. |
| BBa_K4757007 | Composite part of the AlkS-V760E transcription factor controlled by the pEM7 promoter system, with the pAlkB promoter. | BBa_K4757062 | Ps1/Ps2_XylS-K38R-L224Q_Pm-RBS1-mKate2 Construct used to further characterize XylS-mt |
| BBa_K4757063 | pEM7_XylS-K38R-L224Q_Pm-RBS1-mKate2 Construct used to further characterize XylS-mt |
| BBa_K4757064 | Ps1/Ps2-XylS/Pm-RBS1-SPpstu-FAST-PETase Construct used for FAST-PETase expression and secretion |
| BBa_K4757065 | Ps1/Ps2-XylS/Pm-RBS1-SPpstu-FAST-PETase Construct used for LCC expression |
| BBa_K4757066 | pEM7-AlkS-V760E/pAlkB-RBS-mKate2. Construct used for AlkS-V760E testing |
| BBa_K4757067 | Ps1/Ps2-XylS_..._Pm-[SapI]-LUZ7T50-pEM7-BBa_J61100-mKate2-degradation tag. Construct used for testing of different sRNA constructs targeting mKate2 and/or the RBS BBa_J61100. Two SapI recogintion sites between the Pm promoter and bi-directional terminator LUZ7T50 allows for scarless cloning of DNA oligo's coding for the sRNAs. The transcription of the sRNAs is controlled by the inducible XylS/Pm promoter system. The fluorescent protein mKate2 is controlled by the constitutive promoter pEM7, a degradation tag was used to shorten the half-life of mKate2 to allow for more dynamic measurements. |
| BBa_K4757070 | Ps1/Ps2-XylS_..._Pm-[SapI]-LUZ7T50-pEM7-BBa_J61101-mKate2-degradation tag. Construct used for testing of different sRNA constructs targeting mKate2 and/or the RBS BBa_J61100. Two SapI recogintion sites between the Pm promoter and bi-directional terminator LUZ7T50 allows for scarless cloning of DNA oligo's coding for the sRNAs. The transcription of the sRNAs is controlled by the inducible XylS/Pm promoter system. The fluorescent protein mKate2 is controlled by the constitutive promoter pEM7, a degradation tag was used to shorten the half-life of mKate2 to allow for more dynamic measurements. |
| BBa_K4757071 | Ps1/Ps2-XylS_..._Pm-[SapI]-LUZ7T50-pEM7-BBa_K4757003-mKate2-degradation tag. Construct used for testing of different sRNA constructs targeting mKate2 and/or the RBS BBa_J61100. Two SapI recogintion sites between the Pm promoter and bi-directional terminator LUZ7T50 allows for scarless cloning of DNA oligo's coding for the sRNAs. The transcription of the sRNAs is controlled by the inducible XylS/Pm promoter system. The fluorescent protein mKate2 is controlled by the constitutive promoter pEM7, a degradation tag was used to shorten the half-life of mKate2 to allow for more dynamic measurements. |