Vitamin B2, also known as riboflavin, is one of the most
important vitamins. Vitamin B2 deficiency can lead to
inflammation and organic disorders of the mouth, lips, skin, and
genitalia. Despite the importance of vitamin B2 for maintaining
optimal health, there appears to be a lack of awareness
regarding vitamin B2 deficiency among the general population. In
China, the vitamin B2 deficiency rate is as high as 88.1% in
males and 85.4% in females, and most of them are not aware of
it. To deal with the prevalence of vitamin B2 deficiency, we
decided to genetically modify the baker’s yeast to make
vitamin-B2-rich bread and steamed buns. Our project aims to
achieve a high yield of vitamin B2 in Saccharomyces cerevisiae by overexpressing GTP synthetase (ADE4) and vitamin B2
synthetase (RIB1 and RIB7). Our experiments demonstrated
technical success, leading us to formulate our business plan. We
plan to establish a company that specializes in selling baking
mixes (contains flour, engineered yeast, sugar, etc.) We then
analyzed different aspects of our business.
China's bakery market is the second-largest market in the world
and is still growing rapidly. In 2022, the bakery market in
China has a growth rate of 9.7% and reached 285.3 billion
Chinese yuan (39.8 billion US dollars). The market is expected to keep growing at a rate of about 7%
for years (iiMedia Research Group.)
Figure 1. A. Market scale and forecast of China’s bakery
industry, 2018-2025. Data for 2023-2025 are estimated values
(iiMedia Research Group.) B. Baking Mix Market Size in China,
2015-2021 (Huajing Industrial Research Institute.)
Unlike commercial baking, which has attracted a large amount of investment, home baking
is still in its early stages, and there is still a lot of room
for new companies. In China, the home baking market has more than 800 million
consumers. When interviewing Prof. Huang from the College of Food Science and Light Industry, Nanjing Tech University, he recommended baking mix as
a new trend in the bakery industry. According to Huajing Industrial Research Institute, in 2021, the baking mix market in China reached 2.2 billion Chinese
yuan (0.31 billion US$). We believed in the prosperity of the baking mix industry
and decided to go into this business.
By mixing our engineered S. cerevisiae dry powder, with flour and other ingredients, we
developed baking mixes for homemade vitamin B2-enriched food.
For a product demonstration, we design the package of our product based on existing products (Figure 2A). Since we do not have the product yet, we used the baking mix from Xinliang for this demonstration (Figure 2B). We successfully made bread with the baking mix (Figure 2C).
Feature 1: Overproduction of vitamin B2 in yeast
By overexpressing ADE4, RIB1, and RIB7 in S. cerevisiae, we created a yeast strain with a 91% increase in vitamin B2
production. Our baking mix contains this engineered yeast,
enabling the production of vitamin B2-enriched food.
Feature 2: Suitable for various food
To broaden the market, we plan to develop different series of
baking mixes for different target consumer groups. The following
is our product line.
1. Homemade Bread Series:
Baking mix for common bread: suitable for toasts, bagels, baguettes, etc.
Baking mix for whole-wheat bread: whole-wheat flour added.
Baking mix for flavored bread: flavors (chocolate, vanilla, etc.) added.
2. Homemade Dim Sum Series:
Baking mix for steamed bun-based food: suitable for Chinese steamed buns, baozi, Mandarin rolls,
etc.
Baking mix for fried dough sticks: fried dough sticks require a special mix.
3. Special Series:
Low-sugar/sugar-free baking mix: specially designed for
diabetics and consumers trying to lose weight.
Gluten-free baking mix: specially designed for people with
gluten intolerance.
Calcium-fortified baking mix: specially designed for children
and the elderly.
4. Children Series:
Colorful baking mix: natural pigments are added, enabling the
production of colorful food. Designed to increase children’s
interest and appetite.
Prices of current baking mix products in China range from about
10 to 25 Chinese yuan/kilogram. The prices are determined by
manufacturer and use. Generally, baking mixes for bread are more
expensive than that for steamed buns. Our products have a
special advantage, which is the capacity of making vitamin
B2-enriched food. This advantage could help us increase the
prices. Through the analysis, we determined the appropriate
prices for our products.
The price of the Homemade Bread Series is set to 30 Chinese
yuan per kilogram and the price of the Homemade Dim Sum Series
is set to 15 yuan. The price of the Special and Children Series should be higher than that of the Homemade Bread
Series, which is set to 40 yuan.
Manufacturer |
Used for what food |
Price per kilogram (Chinese yuan) |
Shangyidao |
Steamed bun |
15.2 |
Xinliang |
Steamed bun |
12.7 |
Jinshahe |
Steamed bun |
12.9 |
Welna |
Scone |
23.9 |
Welna |
Rice bread |
25.5 |
Songyan |
Mochi |
19.2 |
Rongsheng |
Cream Puff |
35 |
Table 1. List of popular baking mixes in China.
Strengths:
Technical advantages: the unique synthetic biology method
enables yeast to produce a large amount of vitamin B2 during
fermentation.
Health orientated: meet the growing demand of consumers for
healthy food.
Diversified product line: covering the needs of different
consumer groups.
Weaknesses:
High start-up cost: the research on synthetic biology may
require high investment.
Consumer acceptance: consumers may have concerns about
genetically modified yeasts.
Opportunities:
Healthy food market growth: as people pay more attention to
health, the healthy food market has great growth
potential.
Partners: cooperate with dietitians, gyms, and government to promote our products.
International market: In addition to the local market, you can
also consider exporting products to other countries and
regions.
Threats:
Competitors: some companies such as Xinliang have captured a certain market share. Other companies may employ
engineered yeast as well.
Regulatory risks: Regulations on GM products may become
stricter.
As food made with genetically modified organisms, it was meant
to be a special product that acquired unusual restrictions. We
did literature research to find the regulations and evaluation
processes for engineered organisms and new food raw materials.
To conclude, the vitamin-B2-enriched food made with our yeast
has to go through two rounds of assessment. The yeast’s safety
as a genetically modified organism will be evaluated first.
After approval, we can move to the next step, which is to
evaluate the safety of our yeast as a food material. After that,
we can use our yeast to make bread and buns and sell them in
supermarkets. Although complicated, the yeast and food can be
approved if we comply with all the regulations
1. Our yeast must pass three stages of trials: intermediate test,
environmental release test, and production test (the Measures
for the Administration of the Safety Evaluation of Agricultural
Genetically Modified Organisms (2016 Revision, Article
13).
2. The State Commission for the Safety of Agricultural Genetically
Modified Organisms and the Office for the Safety Management of
Agricultural Genetically Modified Organisms are responsible for
the general safety evaluation of our yeast, and we must submit
the Application for the Safety Evaluation.
3. The Ministry of Agriculture and Rural Affairs is responsible
for issuing the Agricultural Genetically Modified Organisms
Safety Certificate.
4. The Chinese National Center for Food Safety Risk Assessment is
responsible for the safety evaluation of our yeast as a food raw
material, and we must submit the Application for the Evaluation
of New Food Raw Materials (Administrative Measures for the
Safety Review of New Food Raw Materials (2017 Amendment),
Article 6).
5. The Chinese National Center for Food Safety Risk Assessment
will publish the Consultation Paper on our yeast
(Administrative Measures for the Safety Review of New Food Raw
Materials (2017 Amendment), Article 6).
6. The National Health Commission is responsible for issuing the
Approved List of New Food Raw Materials.
1. Market Research: Year 0-1.
Research the market. Analyze competitors. Understand industry trends and regulatory requirements.
2. Recruitment and training: Year 0-5
Determine the core team structure of the company. Recruit key positions. Design employee training procedures
3. Fundraising: Year 1-6
Make budget plans and financial forecasts. Look for potential investors or partners.
4. Product development: Year 0-2
Optimize genetically engineered yeast. Develop and test different baking mixes.
5. Product approval: Year 2-3
Get the GMO and the GM food approved.
6. Product promotion: Year 4-10
Create an attractive brand image. Formulate preliminary marketing strategies, such as
advertising, social media promotion, etc. Establish communication channels with consumers.
7. Supply chain and production: Year 4-10
Evaluate raw material suppliers and establish a stable
cooperative relationship. Design the production process to ensure efficiency. Invest in equipment.
Figure 3. Project’s timeline by years.
1. Management team:
Chief Executive Officer (CEO): Responsible for the company's
overall strategy and important decisions.
Chief Financial Officer (CFO): Responsible for financial
management, budget, and investment strategy.
2. Research and Development Department:
Responsible for microorganism and product research. Ensures
that the techniques match the company's business goals.
3. Production and Supply Chain Department:
Responsible for production management, inventory management,
and quality control. Establishes and maintains relationships
with suppliers.
4. Marketing and Sales Department:
Responsible for making marketing strategies. Responsible for
advertising and brand building. Establish relationships with customers.
5. Customer Service and Support Department:
Provide market support and customer service. Collect customer feedback and cooperate with other departments
to improve products.
6. Human Resources Department:
Responsible for recruitment, employee training, and employee
management. Ensures employee satisfaction.
7. Finance and Administration Department:
Responsible for daily affairs, budget, and investment
strategy. Ensure the company's compliance and handle legal and
administrative problems.
1. Seed round:
Target: US$200,000.
Main sources: start-up incubators, personal relationships
(family and friends)
Expected time: year one.
2. Angel round:
Target: US$1,000,000.
Main source: angel investor, professional financing
platform.
Expected time: year three, after product approval.
In the early stage of the company, the budget is often limited,
so it is necessary to adopt efficient and low-cost methods to
promote our products.
Social media:
Pay for targeted advertising to accurately target the
customer.
Reward customers who leave comments about our products on
social media.
Register accounts on platforms (Xiaohongshu, Instagram, WeChat,
Weibo, etc.) and create attractive content, such as cooking
videos and recipe sharing.
Partnership:
Cooperate with baking influencers on social media to promote
our products.
Cooperate with bakery schools, online stores, and
supermarkets.
Provide free tasting in supermarkets.
Brand building:
Reward customers who leave comments about our products on
social media.
Provide free tastings in supermarkets.
Membership system:
Reward customers for membership.
Regularly send news, offers, and recipes to members.
References
Administrative Measures for the Safety Review of New Food Raw
Materials (2017 Amendment PKULAW Version),
lawinfochina.com/display.aspx?id=28054&lib=law. Accessed 27
Sept. 2023.
Huajing Industrial Research Institute. China Baking Mix Industry Market Status and Investment
Strategy Research Report, 2022-2027, https://www.sohu.com/a/630656578_120928700. Accessed 10 Oct. 2023.
iiMedia Research Group. Research Report on the Current Situation and Trend of Chinese
Bakery Industry in 2023, www.iimedia.cn/c400/91410.html. Accessed 10 Oct.
2023.
Measures for the Administration of the Safety Evaluation of
Agricultural Genetically Modified Organisms (2016 Revision),
www.lawinfochina.com/display.aspx?id=24070&lib=law&EncodingName=gb2312.
Accessed 25 Sept. 2023.
Regulation on Administration of Safety of Agricultural
Genetically Modified Organisms of China (2017 Revision),
pkulaw.com/en. Accessed 25 Sept. 2023.