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BBa_K4678006 Description：

To construct the dcs-curs fusion gene, the stop codon of DCS was removed and a three amino acid linker (Gly-Ser-Gly) was introduced between the open reading frames of DCS and CURS , which is sited directed-mutagenesis through two rounds of PCR. What's more The results of bioinformatics indicated that the two proteins had stronger interaction. There are many interacting amino acids and salt Bridges between fusion proteins, which maintain the stability of fusion proteins.

BBa_K4678007 Description：

First of all, the core area of PsrfA is reformed. Replacing the -10, -15, and -35 regions with siga-dependent promoter conserved sequences produced A series of variants.Only P1, where the -35 region is replaced by a conserved sequence, produces the highest RFI. The promoter PS1E is obtained by replacing this position sequence of the most robust variant PS1 with CTG.PS1E is 1.65 times more powerful than the strongest natural promoter PlytR. We constructed DCS-CURS fusion protein , which can improve the expression stability and expression of the two genes. In addition, a curcuminoid-producing unnatural fusion protein diketide-CoA synthase-curcumin synthase (DCS-CURS) was constructed. This can improve the stability of the protein and increase the expression of the protein. Under the strong promoter, the sensitivity which regulating the expression of fusion proteinl is further improved.