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Project Description



According to data from the Ministry of Environment (2015), more than 7.5 million tons of solid waste are produced annually in Peru, 64% of which originate from households, the rate increasing exponentially over time. In addition, according to World Bank projections, daily garbage production could reach up to 36,000 tons by 2025 (WWF Perú, 2018) . In a year, approximately 30 kg of disposable plastic is consumed by the average Peruvian population, given that the country lacks regulations, excess plastic is followed by dire consequences (Responsible Travel Peru 2022). Hence, our team is working on modifying Escherichia coli to digest polyethylene terephthalate (PET) utilizing the enzymes PETase and MHETase. To accomplish this, our focus is to design a construct that changes the by-product of ethylene glycol into glycolate, which can then be metabolized into ethanol as the final result. In order for this to occur, a variety of plasmid developed constructs that incorporate both PETase and MHETase are in development. For this, the fucO and aldA genes present will be over expressed in the construct created, converting ethylene glycol to glycolate. It is anticipated that our bacteria will be able to break down PET plastic to ultimately produce ethanol for use as a biofuel with the help of the PETase and MHETase plasmids in order to perform the transformation. Degrading waste plastic utilizing this process allows for the creation of a non-toxic biodegradable biofuel, ethanol. If implemented, this method will prevent further issues regarding some aspects of Peru's economy by finding ways to reduce plastic waste and simultaneously benefiting the environment. The creation of this biofuel in this precise way with the help of synthetic biology is crucial for transportation, electric and heating sources, while still benefiting the environment by reducing greenhouse gas emissions released by waste. With this, the less-advantaged communities in Peru could get provided with energy-required services/products that they currently cannot afford, and would have a cleaner environment to sustain their lives.