Parts
Overview
As part of the ICARUS system, we decided to build a construct with the most optimal secretion. To achieve this, we decided to test different combinations of signal peptides, promoters and cargos. In this process, we aim to select a combination that will secrete soluble cancer antigens with the highest secretion efficiency possible. As such, we have constructed and utilized a variety of basic and composite parts in the build-up of our system.
Basic Parts
Our team selected multiple signal peptides for secretion of proteins in mammalian cells. Through literature search we identified most efficient signal peptides and decided to study them. In addition to these parts, we selected the HA tag (BBa_K1150016) and BM40 (BBa_K2170214), which both are reported in the registry.
| Part Name | Description |
|---|---|
| K4646000 | HSA |
| K4646001 | IFN |
| K4646002 | Human trypsinogen - 2 |
| K4646003 | Ig Kappa |
| K4646004 | IL-2 |
| K4646005 | T-Cadherin |
Composite Parts
As our end goal, we want to create a library of functional expression units that are capable of secreting protein cargos of choices with desired secretion efficiency. There is a number of composite parts that we have not yet tested, so we refrained from including them in this list until we gather more reliable data on their use.
| Part Name | Description |
|---|---|
| K4646008 | CMV_Kozak_BM40 |
| K4646009 | CMV _Kozak_HA sequence |
| K4646010 | CMV _Kozak_Human trypsinogen - 2 |
| K4646011 | CMV _Kozak_Ig Kappa |
| K4646012 | CMV _Kozak_IL-2 |
| K4646013 | CMV _Kozak_T-Cadherin |
| K4646014 | EF1alpha _Kozak_BM40 |
| K4646015 | EF1alpha _Kozak_HA sequence |
| K4646016 | EF1alpha _Kozak_Human trypsinogen - 2 |
| K4646017 | EF1alpha _Kozak_Ig Kappa |
| K4646018 | EF1alpha _Kozak_IL-2 |
| K4646019 | EF1alpha _Kozak_T-Cadherin |
| K4646020 | SV40_Kozak_HA |
| K4646021 | SV40_Kozak_Trypsinogen |
| K4646022 | SV40_Kozak_BM40 |
| K4646023 | SV40_Kozak_IL2 |
| K4646024 | SV40_Kozak_Tcadherin |
| K4646025 | HA-sfGFP |
| K4646026 | HSA-sfGFP |
| K4646027 | IFN-sfGFP |