Part Collection
All parts we used/planned to use in our project can be found here.
Custom Plasmid backbone
- BBa_K4706000 pSB1C30YIpHR-HO Chromosomal integration at HO loci
Promoters we identified in S.cerevisiae genome
- BBa_K4706002 PHO5 phosphate repressed promoter
Reporters
- BBa_K4706005 meffRed/meffRFP codon optimized for S. cerevisiae
- BBa_K4706008 eechGFP1 codon optimized S.cerevisiae
Protein Tags
- BBa_K4706010 SV40 NLS codon optimized S. cerevisiae
Cell death inducing proteins
- BBa_K4706007 AtBAG6 induced S. cerevisae cell death
Protein binding region
- BBa_K4706003 SrpR binding region
DNA binding protein
- Basic Part: BBa_K4706009 SrpR codon optimized S. cerevisiae
- Composite part: BBa_K4706021 SrpR SV40NLS for S. cerevisiae expression, gets located to the nuclei
Composite parts
- BBa_K4706012 SrpR repressable PHO5 phosphate level sensor
- BBa_K4706013 Gal1 induced RFP A2 AtBAG6
- BBa_K4706015 SrpR repressable PHO5 phosphate dependent cell death
- BBa_K4706016 CUP1-GFP 2A SrpR-SV40NLS codon optimised for S. cerevisae
Assembled constructs inside appropriate backbone
- BBa_K4706017 pSB1C30YIpHR-HO SrpR-PHO5 phosphate sensor
- BBa_K4706018 pSB1C30YIpHR-HO Gal1 induced RFP A2 AtBAG6
- BBa_K4706019 pSB1C30YIpHR-HO SrpR-PHO5 phosphate dependent cell death
- BBa_K4706020 pSB1C30YIpHR-HO CUP1-GFP 2A SrpR-SV40NLS