Parts

Category Name Biobrick # Origin Engineering Decisions References Phase
Promoters Gmubi BBa_K4201000 Glycine Max (Soybean) 1 Complete, Tested
Whey Proteins Coding Sequences Alpha Lactalbumin BBa_K4201022 Bos Taurus
  • The bovine export signal sequence in the alpha-lactalbumin protein sequence was replaced with the glycine max export signal sequence.
  • Six histidine amino acids were added to the end of the amino acid sequence for western blot analysis.
  • Lastly, codon optimization was used to maximize expression specific to soybeans.
 2 Complete with P + T
Beta Lactoglobulin BBa_K4919000 Bos Taurus
  • The bovine export signal sequence in the alpha-lactalbumin protein sequence was replaced with the glycine max export signal sequence.
  • Six histidine amino acids were added to the end of the amino acid sequence for western blot analysis.
  • Lastly, codon optimization was used to maximize expression specific to soybeans.
 3 Complete with P + T
Reporter Genes GUS BBa_K4201010 Multiple Sources
  • Codon optimized for G. max
 4 Complete with P + T, Tested
Terminators AtHSP BBa_K4201009 Arabidopsis thaliana
  • Chosen for its small genomic size
 5 Complete for use
Backbones Amp BsaI BBa_K4201013 NA
  • Blue/white gene occupying insert position
  • Ampicillin/Carbenicillin Resistance
  • BsaI and AarI/PaqCI Recognition Sites
  • E. coli origin of replication
 - Complete for Use
Chlor AarI BBa_K4201014 NA
  • Blue/white gene occupying insert position
  • Chloramphenicol Resistance
  • BsaI and AarI/PaqCI Recognition Sites
  • E. coli origin of replication
 - Complete for Use
Kan BsaI BBa_K4201015 NA
  • Blue/white gene occupying insert position
  • Kanamycin Resistance
  • BsaI Recognition Sites
  • E. coli origin of replication
 - Complete for Use
Final Composite Parts Gmubi-HSPt-alac-Gmubi-GUS-HSPt BBa_K4201024
  • See Basic Parts
 - In Testing
Gmubi-HSPt-blac-Gmubi-GUS-HSPt BBa_K4919001
  • See Basic Parts
 - In Testing

References

  1. De La Torre, C. M. & Finer, J. J. The intron and 5’ distal region of the soybean Gmubi promoter contribute to very high levels of gene expression in transiently and stably transformed tissues. Plant Cell Rep. 34, 111–120 (2015).
  2. LALBA - Alpha-lactalbumin—Bos taurus (Bovine) | UniProtKB | UniProt. (n.d.). Retrieved October 11, 2023, from https://www.uniprot.org/uniprotkb/P00711/entry
  3. LGB - Beta-lactoglobulin—Bos taurus (Bovine) | UniProtKB | UniProt. (n.d.). Retrieved October 11, 2023, from https://www.uniprot.org/uniprotkb/P02754/entry
  4. Jefferson, R. A. The GUS reporter gene system. Nature 342, 837–838 (1989).
  5. Nagaya, S., Kawamura, K., Shinmyo, A. & Kato, K. The HSP Terminator of Arabidopsis thaliana Increases Gene Expression in Plant Cells. Plant Cell Physiol. 51, 328–32 (2010).
  6. Lebendiker, M. & Danieli, T. Purification of Proteins Fused to Maltose-Binding Protein. Methods Mol. Biol. Clifton NJ 1485, 257–273 (2017).