Wet Lab: The subteam began to brainstorm, and each member developed their own project idea. They familiarized themselves with the role of Wet Lab within the team and became acquainted with the lab space. Learned about iGEM competition, how it works, how the Giant Jamboree works/takes place, and stated their interests (genetic engineering, modeling, writing, etc) on a Google Form

Product Development: The subteam conducted their first meeting, reviewing member expectations and the grading structure for those taking the project team “class” for credit, cell biology, and new member training. Members began participating in the team-wide brainstorming process, each developing their own project idea.

Policy and Practices: The subteam had their first meeting where we discussed the role of PnP within the team and discussed expectations for the semester. New members were given their first assignment of training. Members were required to research previous teams who were listed as model examples for IHP and evaluate their work, including what impressed them, areas that would have approached differently, and more. Members were introduced to brainstorming teamwide and everyone began developing their own project idea.

Wiki: The subteam had their first meeting where they went over grading, an overview of the coming year and team member expectations, and received their first onboarding project. Learned about HTML and CSS basics and discussed key aspects of a good website layout and design.

Business: The subteam met to discuss the importance of commercialization and entrepreneurship in a season-based competition such as iGEM. Criteria for Best Supporting Entrepreneurship was reviewed to instill a sense of direction in the subteam. Guidance over registration was issued. The subteam read the first two chapters of the business plan manual developed by previous Cornell iGEM Business teams. The subteam discussed the application of the readings to potential projects for the coming year.

Wet Lab: Wet Lab continued to brainstorm, narrowing down on the more impactful and feasible project ideas. New members learned how to use PCR to amplify DNA and perform gel electrophoresis to analyze the results of their PCR. They also received an equipment walkthrough of the lab.

Product Development: Members continued to brainstorm, refining the team’s more feasible and impactful ideas. New members learned how to work with LTspice via an online tutorial.

Policy and Practices: New members were given their second assignment of training where they were required to research a previous Cornell iGEM project idea and develop a set of interview questions for it. They were then asked to interview the PD and/or WL leads as a mock interview in order to get more comfortable with the interview process, start to finish, and get some feedback from the interviewees. Members continued to brainstorm as a whole team, developing their ideas.

Wiki: Subteam members continued to work on their first assignment, where they were promoted to redesign the homepage from the 2018 Cornell iGEM wiki. Project had to use a different color scheme and layout, include a nav bar that has a different way of organizing the content, and change 2-3 images. Returning members had to make it mobile responsive and add CSS transitions or JavaScript animations

Business: The subteam finished reading the iGEM Business Plan document and turned in reflections on what they learned from reading the document, what they knew, and what they were excited to learn about entrepreneurship in a synthetic biology/iGEM Jamboree context.

Wet Lab: Wet Lab was introduced to crucial software such as SnapGene, Benchling, NEBioCalculator, etc while learning about the sequence design process and engineering design proces.Presentation delivered on design principles (in vitro, in vivo, in silico), and the importance of wet lab’s role in overall experimental design

Product Development: New members received in-person training on circuitry, including high and low pass circuits, and how to implement a breadboard. Members began developing the engineering implementation behind the final designs in the brainstorm process.

Policy and Practices: New members were given their third assignment of training where they were told to research and write about two areas: bioethics, legislation, or safety and risk assessment in relation to a brainstorming topic of their choice.

For brainstorming, now that projects had been narrowed down, members began focusing on developing more PnP specific work, including reaching out to experts for technical assistance.

Wiki: Subteam had their second subteam meeting where they went over each member’s website redesign of assignment 1. Each member discussed the reasoning and motivation behind their redesign as well as gave feedback to each other. Learned about design psychology and the basics of color theory and graphic design. Subteam members were introduced to Figma and the second onboarding project focusing on design.

Business: In order to ensure the general and subteam’s tasks were achieved fairly, the subteam divided up into 3 subgroups, with a group working on case reviews, sponsorship outreach, and grant research. The case review team had two members make a presentation on prior winners of Best Supporting Entrepreneurship and take questions from the subteam. The sponsorship outreach team brainstormed potential sponsors to fund our project through monetary donations or in-kind donations. The grant research team began compiling a list of applicable grants

Wet Lab: February break, no lab work was performed.

Product Development: Members continued to refine project ideas for the 2023 season.

Policy and Practices: Members continued to reach out to interviewees. Some members began conducting interviews for the remaining brainstorming projects in order to gain a better understanding of the feasibility and necessity of each project. Sciencenter event was planned for the following week, so members began preparing for the event including pre-running the activities to ensure they would run well and buying the appropriate materials.

Wiki: February break, no subteam meeting. Members continued to work on their project 2.

Business: Each member completed a case study report of previous BSE winners over fall break and worked on completing a sponsorship packet for the upcoming season.

Wet Lab: Performed liquid culture and plate preparation training to prepare for mini-prep and transformation protocols respectively.

Product Development: New members began MATLAB training. Members continued to refine the final brainstorming ideas.

Policy and Practices: Members continued to reach out to interviewees for their assigned brainstorming ideas. On March 4th, we hosted our first outreach event of the year: Sciencenter. We wanted to start early this year to provide us with the opportunity to host another event where we could improve upon the feedback and results of this event. Since we had yet to decide on our final project idea for the season, we hosted three different stations, all focused on general synthetic biology concepts.

Wiki: Members continued to work on their design project to gain more experience with design tools such as Figma. Members were given multiple websites to choose from where they had to (1) replicate this site’s layout in Fimga then (2) redesign the website.

Business: Four members presented their case studies to the subteam and took questions from the remaining members. In preparation for our giving day event in March, the remaining members continued building out a sponsor reachout sheet.

Product Development: New members finished MATLAB training while working in collaboration with other members. New members began CAD training in Fusion 360 via a virtual tutorial.. Members continued to refine brainstorming ideas.

Policy and Practices: Members continued to conduct interviews and reach out for future interviewees for their assigned brainstorming project. They reached out to stakeholders this week in addition to technical interviews.

Wiki: Each member presented their design project and explained the motivation behind their design changes. Each team member gave feedback to each other. Started discussing merch. What type of team merchandise we wished to create this year, and what type of designs. Subteam members worked on each designing a merch design for the team.

Business: Our subteam created a 300 character template for linkedin messaging representatives of different companies that were in our sponsor list. Our subteam filed an application to join the Cornell Engineering Innovation Competition on 3/24. Our subteam learned mail-merging techniques to prepare for giving day

Wet Lab: Performed more gel electrophoresis training using old parts from last year’s MicroMurals project.

Product Development: Members continued refining potential project ideas by responding to team feedback. Members wrapped up CAD training.

Policy and Practices: Members began working on lesson plans and slideshows for upcoming Splash! education event. This consisted of researching various areas of synthetic biology research that have serious ethical implications and developing questions and prompts for students to have debates. Members also attended training sessions in preparation for Splash! event. This consisted of receiving training on how to work with minors training and boundaries training for educators through Cornell University’s CULearn online platform. Members also went to training specific to the event hosted by the event coordinators about logistics and safety plans for the day.

Wiki: Subteam learned about Javascript and JQuery. Team voted on merch designs. The one which was chosen was completed in more detail by the subteam member.

Business: Our subteam was focused on contacting donors via linkedin, emails, and mail-merge in anticipation of our giving day event on 3/16/23. Our successful efforts led to our team raising a record $5,579.24

Wet Lab: Performed transformation training using the plates prepped on March 4th (the protocol was successful, all plates had transformed colonies that produced asPink chromoprotein)

Product Development: ENERGEM was chosen as the final project idea for the 2023 competition. All members gathered for a meeting discussing in depth the ENERGEM development process, especially the details surrounding enzyme encapsulation, Ansys modeling, and the bioreactor. New members learned how to implement arduino circuits and continued to refine their breadboarding skills.

Policy and Practices: ENERGEM was selected as the final project. The primary concern for PnP is getting a better understanding of the areas in which 7-methylxanthines can be applied. Our preliminary research indicated that it could have important implications for agriculture or respiratory diseases. Members began reaching out to experts and stakeholders in these fields to gain a better understanding of the applications of 7-methylxanthines. Conducted an interview with Dr. Li. She recommended that we focus on enzymatic efficiency when selecting our pathway.

Wiki: Continued to learn about JavaScript. Team was assigned their final onboarding project where they were given a few pages from past iGEM teams to redesign, implementing the following features: create text over an image upon hovering over it, implement showing and hiding a div upon clicking on it, implement a feature when hovering over text/link, button changes color upon clicking on it

Business: Our team analyzed our 2017 BSE winning business plan and focused on preparing our Engineering Innovation competition slide deck using those insights

Wet Lab: With spring break approaching, no lab activity was performed. The focus of the subteam was finalizing brainstorming details to choose Team Cornell’s 2023 project.

Product Development: Subteam members learned about their role in helping the team meet all medal criteria, and each individually researched a different criteria.

Policy and Practices: Members continued to reach out to stakeholders in both agricultural and medical fields to gain a better understanding of its applications. Interview with Dr. Duplais indicated that agriculture would be a possible application; however, current pesticides are far more effective and that 7-methylxanthines would not be the appropriate class of methylxanthines to produce. Recommended we explore medical and dietary supplement applications instead.

Wiki: Subteam members continued to work on their final onboarding assignment, which would be due the week after spring break.

Business: Subteam members completed an assignment defining their understanding of ENERGEM and listed potential grant funding sources unique to ENERGEM

SPRING BREAK

Wet Lab: A joint meeting with product development was conducted to discuss the finer details of the 2023 project, ENERGEM, after it was voted on in group meeting. Encapsulation, ADE cycle, modeling, and reactor design/testing aspects were discussed and improved upon.

Product Development: Individual medal criteria research was presented at the team meeting. All new and old project materials were moved into the new lab in Ward Hall. The subteam met and discussed potential uses for seven-methylxanthines, as well as where the caffeine reactant can be sourced from.

Policy and Practices: Conducted interview with Dr. DeLisa, who recommended that we make purity a focus of our project if we wanted to choose a medical application. We had an interview later with Dr. Alibi, who confirmed what Dr. DeLisa said. Members brainstormed a list of teams that we could possibly reach out to for collaboration based on their previous projects and experiences, as well as our relationship with teams from previous years. Members began to plan out the rest of the season based on medal criteria and the information from this year’s project.

Wiki: Subteam members continue to work on their JavaScript/JQuery project. With ENERGEM selected as our project, each subteam member was tasked to design a logo for the project, to be voted on by the entire team.

Business: Subteam members were assigned specific business plan aspects for ENERGEM to focus on and were responsible for brainstorming what their sections could like for ENERGEM

Wet Lab: Due to high conflicts, lab work was canceled this week; instead, literature review was the primary focus for Wet Lab.

Product Development: Wet Lab and Product Development met to discuss the reaction process and the logistics behind future collaboration. Each member was assigned research surrounding either the bioreactor housing material or enzyme encapsulation methods.

Policy and Practices: Members began developing infographics for social media and publicity. The first one was on methylxanthines and chocolate to align with the timing of the team’s chocolate fundraiser. Members attended Splash! where we taught a class of bioethics, teaching students about synthetic biology and iGEM and hosting mini debates on different ethical issues in the field.

Wiki: Final logo design was selected by voting from the entire team and will be the ENERGEM logo moving forward. Each subteam member showcased their third assignment to the rest of the subteam. Each member gave their feedback for each project.

Business: With the entire business plan sectioned out, members drafted a few sentences under each header.

Wet Lab: Tested alginate encapsulation procedure with brilliant blue dye to help product development begin their encapsulation work. Establishing protocols for this summer’s lab work. Protocols were written for adapted and directed evolution, enzyme immobilization via alginate bead encapsulation. Theory established for epPCR and his-tag affinity chromatography.

Product Development: Members presented their individual encapsulation methods and housing material ideas at the subteam meeting. Members determined that some form of beading (likely sodium alginate beads) would be most effective in encapsulating enzymes. Members began developing CAD models that would immobilize these beads.

Policy and Practices: Conducted an interview with Dr. Bishara, a physician who specializes in neonatal pulmonary disease. She said that 7-methylxanthines are no longer treated for asthma and COPD on the pediatric level. She recommended that we research pharmaceutical supply chains and how the COVID-19 pandemic impacted them as a possible avenue of exploration. Members continued to brainstorm possible educational and outreach events for the summer and fall.

Wiki: With onboarding completed, we started planning for our Wiki. Started creating design options for our wiki (theme, color scheme, etc.) to be voted on by the entire team. Subteam members were each teasked to create a homepage banner for our wiki.

Business: An initial rough draft of the respiratory illness based ENERGEM was compiled.

Wet Lab: Wet Lab reviewed the summer plan during the final week of the spring session. No in-lab work was performed.

Product Development: Members finalized their CAD models from the previous week and presented them to the entire team at the meeting. Members brainstormed improvements for the aforementioned CAD models.

Policy and Practices: Members began work on brainstorming ideas for an ENERGEM specific game. Finally decided on a game where players would be given different starting materials to come up with a final product of their own; teaching them the different components and the workflow of an iGEM project. Conducted an interview with Dr. Langer, a clinical pharmacist. She recommended we look at specific drugs with 7-methylxanthines listed as an ingredient. She supported the fact that we should look into COVID-19 supply chains. Conducted an interview with Dr. Milner, who confirmed that our current technical plan was feasible and provided some advice on our questions.

Wiki: Homepage banner chosen by a vote from the entire team. This will be the base of our design theme for the entire website.

Business: Peer review of drafted business plan was conducted, other subteams were contacted for feedback

Wet Lab: Wet Lab was not in session until summer.

Product Development: Product Development did not meet this week.

Policy and Practices: PnP discussed starting up the summer class information including brainstorming possible activities. Since we were still deciding about whether we would host the class in person or online, we were primarily focused on how to navigate a hybrid environment while being as engaging as possible.

Wiki: Created and went over our work timeline for summer break. This included who was going to work on game development (for PnP) as well as those working on the base of the wiki.

Business: A spring 2023 ENERGEM rough draft was completed

FINALS WEEK

BREAK

BREAK

BREAK

Wet Lab: Wet Lab was not in session.

Product Development: Product Development did not meet this week.

Policy and Practices: PnP did not meet this week.

Wiki: Subteam continued to work on assigned summer deliverables including game and website development.

Business: Business team is on break during the summer session

Wet Lab: With the start of the summer, preparatory work was conducted which included inventory, autoclaving materials, and defrosting the freezers.

Product Development: Members inventoried all available materials in the Ward lab.

Policy and Practices: First priority of the summer was to look into marketing for the summer class, which included reaching out to various local middle and high schools as well as schools throughout upstate New York. Given that we were worried about responses as many schools were already out for the summer, we began planning how we would adapt our content to a completely virtual mode. Members began to reach out more interviewers for possible stakeholder interviews to understand more about other avenues for applications of 7-methylxanthines given the interviews at the end of the semester that informed us that they were not as readily used for respiratory diseases any longer.

Wiki: Subteam continued to work on assigned summer deliverables including game and website development.

Business: Business team is on break during the summer session

Wet Lab: Wet Lab began work on PCRing newly ordered parts and attempting Gibson Assembly to acquire our full plasmid. The PCR resulted in low concentrations and the Gibson failed.

Product Development: Jonathan began modeling the bioreactor in ANSYS. Jatin and Sonia began working on the bioreactor. They developed an experimental method for testing residence time. They purchased PVC to house the reactor.They refined CAD models from the spring.Rita and Rachel began working on enzyme encapsulation.They practiced forming beads by dropping sodium alginate solution into calcium chloride solution.They created beads from incrementally increasing concentrations of sodium alginate to test bead properties.

Policy and Practices: Members worked on finalizing the pathways that we would include in the game. They were all based on former iGEM projects. Outline was finalized and sent to the Wiki subteam to start work early on. Given that we received minimal responses to our class, we decided that we would develop lesson plans to post online for people to access if they would like. Members all focused on one specific subteam and their work and came up with possible projects that children could do at home, aligning with the lesson plan.

Wiki: Subteam continued to work on assigned summer deliverables including game and website development.

Business: Business team is on break during the summer session

Wet Lab: Wet Lab conducted some troubleshooting and continued to work on getting the Gibson Assembly to work. Highlighted some potential design issues and re-ordered primers to rectify the issue and potentially raise concentrations. We modeled a basic Poiseuille’s Tube in ANSYS to gain a better understanding of the pressure and velocity gradients of a basic tube. Modeling plan was revised and refined slightly to better accommodate for any hardware changes.

Product Development: Jonathan continued working on ANSYS modeling. Jatin and Sonia continued bioreactor work: They created a mock-up sketch of the reactor design. They began slicing CAD models. They began the process of building a mock reactor. Rita and Rachel continued encapsulation work: They wrapped up analysis of the sodium alginate concentration experiments. They created calcium chloride solutions of different concentrations and began creating beads with each solution. They began brainstorming a test enzyme to use, but Wet Lab’s stock of enzymes that are both cheap and easy to implement is running low. They may wait until Wet Lab produces the actual lysate.

Policy and Practices: Members began reaching out to ethics experts to gain a better understanding of the policy work needed for ENERGEM. Given that the interviews at the end of Spring semester were not very enthusiastic about an application for respiratory disease, we decided to incorporate this expert advice and continue to explore other avenues. Our research indicated myopia, specifically childhood myopia, as a possible area where 7-methylxanthines would be effective, so members began reaching out to ophthalmologists and researchers in the field.

Wiki: Subteam continued to work on assigned summer deliverables including game and website development.

Business: Business team is on break during the summer session.

Wet Lab: With new primers, Wet Lab performed PCR amplifications of inserts and backbones and re-attempted Gibson Assembly.Worked on documentation for an in-house documentation deadline. We added a sphere of finite volume to our ANSYS model, mimicking one encapsulated bead in the tube.

Product Development: Continuing build process of engineering cycle for the reactor: locating 3D printer options for immobilization design. Testing varying calcium chloride concentrations for encapsulation method to determine ratio best suited for our purposes. ANSYS modeling difficulties with lab access

Policy and Practices: Members continued to reach out to stakeholders for possible applications in myopia. Members began working on researching policy aspects to the project, including understanding important legislation, bioethical issues, and safety and risk assessment requirements. Members began evaluating our project using different human practices frameworks, building upon the work of Exeter iGEM. We used ELSA, AREA, and SEG. Members reached out to ethics experts to gain feedback on the framework we developed last year: EUDI. Once we gain advice on our framework, we will apply it to our project.

Wiki: Subteam continued to work on assigned summer deliverables including game and website development.

Business: Business team is on break during the summer session.

Wet Lab: Continued Gibson Assembly attempts with transformation and gel proof of concept. Shifted focus to troubleshooting the protocol - highlighted an issue with our backbone.

Product Development: Completed single bead model in laminar flow conditions, results are confusing and will continue testing other options. Encapsulation method finished testing and 2 combinations of concentrations were determined as most viable, waiting for enzymes to fully test encapsulation constraints. Reactor built with PVC and plastic beads to model future produce encapsulated beads with enzyme.

Policy and Practices: Members brainstormed ideas for our summer experiment video series, which would consist of how-to videos to show experiments to younger kids related to important iGEM concepts. Members began writing scripts for the videos. Members began researching and writing for our methylxanthines application handbook that will include information on all the methylxanthines involved in the caffeine degradation pathway, not just 7-methylxanthines as our work can be applied to producing other chemical products in the pathway, not just 7-methylxanthines.

Wiki: Subteam continued to work on assigned summer deliverables including game and website development. Base design and formatting for wiki was complete.

Business: Business subteam met with Joe Price, CEO of Evolutor, who offered perspectives of how to scale a start-up from an iGEM team (Sheffield) and offered a knowledge exchange of increasing the metabolic rate of our proteins with our sponsorship package.

Wet Lab: Continued troubleshooting with extra positive and negative controls to pinpoint the issue with our backbone.

Product Development: Starting documentation process for all sub-projects. Moving forward with ANSYS model and incorporating turbulent flow dynamics. Encapsulation methods are lengthy: brainstorming potential auto-bead maker options. Finalizing 3D printed immobilization method and scaling as well as procurement of a printer.

Policy and Practices: Members got together to film the videos for the Summer Experiment Series. Team finalized 5 different videos which will cover areas ranging from DNA to polymer chemistry. Members began editing videos to be ready for distribution in mid-August. Members continued to work on our applications handbook.

Wiki: Subteam continued to work on assigned summer deliverables including game and website development.

Business: Business subteam met with Charlie Maddox, CEO of Enzymatic Inc, who advised our team on taking steps to protect our IP and scale our commercial processes.

Wet Lab: PCR amplified a distribution kit backbone source and performed a gel of Gibson Assembly, old, and distribution kit backbone sources to confirm or repute backbone presence. Discovered a potential issue with the DNA cleanup step. Ordered a new pSB1C3 backbone for current and future team use. GenScript free plasmid was ordered after winning the giveaway of the fully proposed plasmid created by CUGEM.

Product Development: Turbulent model is being worked on, one layer meshing needs work as well. Have to rescale immobilization method due to printer constraints - moving forward with testing in a 10 mL syringe tube to model future scale-up designs. Dimensional analysis training for potential reactor calculations Starting large scale encapsulation to produce more beads for testing

Policy and Practices: On August 5th, we hosted our second Sciencenter event of the competition season. We continued with the same events from the last session, just adding an extra station that was specific to ENERGEM. Event at Kendal @ Ithaca was finalized this week after emailing with their event coordinators and sharing an event advertisement to include in their September newsletter.

Wiki: Subteam continued to work on assigned summer deliverables including game and website development.

Business: Business subteam met with PacBio to discuss sponsorship opportunities.

Wet Lab: DNA cleanup troubleshooting to observe the problem of backbone disappearance during Gibson Assembly. Liquid cultures of new pSB1C3 backbone plasmid were created

Product Development: ANSYS one bead model in the works and continuing turbulent flow model. Testing auto bread maker ideas for the encapsulation protocol - most likely using a Harvard Apparatus syringe infusion pump. Waiting for materials to assemble new mock reactor.

Policy and Practices: Continued to finish up editing videos for the summer science experiment video series. Members finished their assigned informational graphics on 7-methylxanthines, the applications of methylxanthines, synthetic biology, and more in preparation for our upcoming event at the Farmer’s Market. Event at Longview Senior Living Community was finalized this week after Zoom meetings with their event coordinators, which included sharing plans for content and discussing scheduling.

Wiki: Subteam continued to work on assigned summer deliverables including game and website development.

Business: Subteam met with representatives from the Office of Technology Licensing to learn more about specific protections that we will need for our project.

Wet Lab: Wet lab on break between summer and fall session.

Product Development: Product Development on break between summer and fall session.

Policy and Practices: Conducted interviews with various stakeholders including ophthalmologists and drug development companies

Wiki: Subteam continued to work on assigned summer deliverables including game and website development. Wiki Mockup complete

Business: Our subteam met with Telesis Bio to discuss sponsorship opportunities and struck a tentative agreement for a BioXP system to be installed on campus.

Wet Lab: Wet lab on break between summer and fall session.

Product Development: Product Development on break between summer and fall session

Policy and Practices: This week we took a break before we hosted our event at the Ithaca Farmers Market on August 20 where we learned a lot from those we spoke to there.

Wiki: Subteam continued to work on assigned summer deliverables including game and website development.

Business: The subteam discussed a new vision for the project given the summer updates of the project. A new table of contents was established and members assigned to it

Wet Lab: Performed Gel Electrophoresis on old NdmAB parts, problem still persists. As troubleshooting, re-PCRed old and new part of NdmABD, performed DNA cleanup, and digested pSB1C3 backbone

Product Development: Finalizing turbulent flow ANSYS model. Syringe infusion pump testing was positive. Attempting to procure mock enzyme for bead encapsulation testing. Building new reactor model with 10 mL syringe replacing existing PVC tube

Policy and Practices: We continued to work on organizing and planning for our upcoming outreach events. Worked with Wiki to finalize what we wanted for the game. Continued to reach out to possible government officials

Wiki: First in person subteam meeting of the semester. Wiki and Game dev both updated on summer progress. Game development was slightly behind schedule but no pressing issues. Constant communication with other subteams about documentation deadlines

Business: Subteam met with Management Library to learn how to effectively leverage Cornell’s resources for more strategic research. Team further outlined vision for 2023 fall business plan

Wet Lab: Nano Dropped all new PCR products. Performed Gibson Assembly of old products. Received new pSB1C3 backbone and began to transform, miniprep, and PCR it.

Product Development: Reactor with syringe was constructed. Immobilization method was scaled down and printing this week. ANSYS model finalized

Policy and Practices: Continued to work on applications handbook. Finalized slideshows for the nursing home events. Started to reach out to the elementary school to organize event at the beginning of October

Wiki: Continued to work on Wiki. Started designing page banners. Created new templates to go with these banners. Worked on promotional video.

Business: Subteam prepared to present our project to the Cornell eLabs accelerator program on 9/5/2023. Business model section was renovated.

Wet Lab: Performed Gibson with new backbone and NdmABD parts

Product Development: Met with Professor Bhaskaran to talk about future ANSYS considerations. Reactor with syringe tested with immobilization method and produced encapsulated beads - need a new testing procedure due to error in size of molecules.

Policy and Practices: We hosted our event at Kendal at Ithaca - used the feedback from this event to work on improving our event at Longview

Wiki: Each subteam member was assigned tasks regarding the website. From design to feature implementation, as well as continue to work with PnP and game dev.

Business: Subteam met with Dr. Zatrenau of Rochester Regional Health to understand how to leverage physcian to patient relationships to boost drug sales; understood FDA strategy and regulation

Wet Lab: As Gibson is worked on, Wet Lab shifted focus to help PD with reactor testing via transforming bacteria with pink chromoproteins. Created glycerol stocks of approximately 15 tubes of chromoprotein expressed E. Coli. Performed Gel Electrophoresis of new parts’ Gibson → failed

Product Development: Discussed with Wet Lab future steps for model of proof of concept of the encapsulation beads. Updating documentation of overall parts of the project. Working on finalizing new ANSYS model after discussing with Professor Bhaskaran how to improve it

Policy and Practices: Hosted our event at Longview Senior Living Community.

Wiki: Found issues with game development. Reverted much of what was created and almost completely started over. Constant communication with other subteams about documentation.

Business: Subteam met with Dr. David Putnam, Dean of Engineering Innovation who taught us the mechanisms of Series A-C funding and how to leverage Cornell’s incubators to turn our project team into a full fledged company

Wet Lab: Updated documentation of wet lab work so far in preparation for Wiki Freeze

Product Development: Waiting for ANSYS model to complete computation. Started testing proof of concept for encapsulation methods using chromoproteins. Cleaning up reactor design to final model for easiest operation.

Policy and Practices: Planned out the remainder of the elementary school. Worked on documentation in preparation for wiki freeze

Wiki: Each member assigned multiple pages to complete implementation of. Continued to have constant communication with other subteams to discuss wiki design and implementation.

Business: Finalized business plan.

Wet Lab: Continued documentation for wiki freeze. Began encapsulation preparations for the bacteria with chromoproteins for PD’s reactor testing

Product Development: Finalized ANSYS model and writing up associated documentation. Continued various encapsulation proof of concept testing

Policy and Practices: Hosted event at the elementary school.

Wiki: Continuous work up until WikiFreeze.

Business: Finished business plan.

Wet Lab: Finalized documentation for wiki freeze. Created plan to continue working on ENERGEM post-wiki freeze.

Product Development: Finalized documentation for wiki freeze. Continued working on scaled-up version of reactor.

Policy and Practices: Finalized documentation for wiki freeze.

Wiki: Finalizing Wiki and Game for WikiFreeze.

Business: Finalized documentation for wikifreeze.