The experimental workflow used during the wet lab part of our iGEM project involved several types of experiments. To get a more detailed overview of the workflow, please refer to the lab notes on theNotebook page.All of the relevant experimental protocols used during our iGEM project can be found below.
Protocols
Plating and liquid culture preparationColony PCR - PHIRE IICompetent E.coli cellsElectroporation Solution (ES)Gel electrophoresisGeneJET Plasmid Miniprep kit Purification (ThermoFischer)Gibson AssemblyGYT mediaL. plantarum competent cells and electroporationL. reuteri electrocompetent cellsShort Guide for Yeast MoClo-WorkflowMRS agarMRS brothOD measurementPHUSION polymerase protocolPrep of MoClo partsQIAprep Miniprep (QIAGEN) E.coliThermoCycler protocol
