We have successfully constructed and tested one new composite part (PETase-R, BBa_K4804000). PETase (PET-digesting enzyme) is secreted by a bacterium (Ideonella sakaiensis 201-F6) that has the ability to degrade PET plastic (Harry P., et al., 2017). Spider silk has high strength and toughness, and has a unique three-dimensional spatial network structure, which can be used as a fixed material for some macromolecules.
Figure 1: Constitution of T7 Promoter-RBS-PETase-R-T7 Terminator gene circuits
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