New parts
New parts are presented by project subarea. Refer to the parts registry
and design pages for more information on the design, results and
references of each part.
PET depolymerization
See additional notes on the design
here.
| Name |
Registry ID |
Description |
Length (bp) |
| pelB-FAST-PETase |
BBa_K4701300
|
Extracellular FAST-PETase expression |
882 |
| lamB-MHETase |
BBa_K4701301
|
Extracellular MHETase expression |
1866 |
| SpyCatcher002-FAST-PETase |
BBa_K4701302
|
FAST-PETase/MHETase fusion |
1152 |
| SpyTag-MHETase |
BBa_K4701303
|
FAST-PETase/MHETase fusion |
1821 |
| Name |
Registry ID |
Description |
Length (bp) |
| pelB signal sequence with g58a |
BBa_K4701200
|
pelB variant for FAST-PETase secretion from E. coli
|
66 |
| lamB signal sequence |
BBa_K4701201
|
lamB signal sequence for MHETase secretion from
E. coli
|
81 |
| FAST-PETase |
BBa_K4701202
|
Coding sequence for an IsPETase variant |
789 |
| MHETase |
BBa_K4701203
|
Coding sequence for WT MHETase |
1758 |
| SpyCatcher002 |
BBa_K4701204
|
Coding sequence for SpyCatcher002 with a GlySer-linker |
363 |
| SpyTag |
BBa_K4701205
|
Coding sequence for SpyTag with a GlySer-linker |
63 |
EG assimilation
See additional notes on design
here.
| Name |
Registry ID |
Description |
Length (bp) |
| fucO |
BBa_K4701210
|
Coding sequence for fucO |
1149 |
| aldA |
BBa_K4701211
|
Coding sequence for aldA |
1440 |
TPA assimilation
See additional note on design
here.
| Name |
Registry ID |
Description |
Length (bp) |
|
tpaK-tph operon (High transcription)
|
BBa_K4701306
|
Operon with 5 coding sequences for TPA assimilation, downstream
of a strong promoter.
|
5392 |
|
tpaK-tph operon (Medium transcription)
|
BBa_K4701307
|
Operon with 5 coding sequences for TPA assimilation, downstream
of a medium promoter.
|
5392 |
|
tpaK-tph operon (Low transcription)
|
BBa_K4701308
|
Operon with 5 coding sequences for TPA assimilation, downstream
of a weak promoter.
|
5392 |
| Name |
Registry ID |
Description |
Length (bp) |
| tpaK |
BBa_K4701220
|
Coding sequence for tpaK |
1380 |
| tphA1 |
BBa_K4701221
|
Coding sequence for tphA1 |
1011 |
| tphA2 |
BBa_K4701222
|
Coding sequence for tphA2 |
1242 |
| tphA3 |
BBa_K4701223
|
Coding sequence for tphA3 |
465 |
| tphB |
BBa_K4701224
|
Coding sequence for tphB |
948 |
| RBS for tpaK |
BBa_K4701230
|
Synthetic RBS upstream of tpaK |
25 |
| RBS for tphA2 |
BBa_K4701231
|
Synthetic RBS upstream of tphA2 |
25 |
| RBS for tphA3 |
BBa_K4701232
|
Synthetic RBS upstream of tphA3 |
25 |
| RBS for tphB |
BBa_K4701233
|
Synthetic RBS upstream of tphB |
25 |
| RBS for tphA1 |
BBa_K4701234
|
Synthetic RBS upstream of tphA1 |
25 |
| Stabilizing hairpin |
BBa_K4701235
|
mRNA hairpin for stabilizing secondary structure around the
first RBS sequence
|
18 |
Reused parts
We also re-used some parts from the registry in our designs.
| Name |
Registry ID |
Description |
Length (bp) |
| T1 Terminator |
BBa_K3675003
|
T1 Terminator for P. putida KT2440 we used in the
tpaK-tph operon parts.
|
158 |
| Strong constitutive promoter |
BBa_J23102
|
Used in the high transcription tpaK-tph variant.
|
35 |
| Medium constitutive promoter |
BBa_J23105
|
Used in the medium transcription tpaK-tph variant.
|
35 |
| Weak constitutive promoter |
BBa_J23114
|
Used in the weak transcription tpaK-tph variant.
|
35 |