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Experiments
Cloning Protocols
Colony PCR Protocol
Dpni Digestion of PCR Products
Drop Dialysis
Gel Run Protocol
Gel Validation and Extraction Protocol
Gibson Assembly Protocol
Golden Gate Assembly Protocol for Using Nebridge Golden Gate Assembly Kit Bsai Hf V2 E1601
Heat Shock Transformation for E Coli 10 Beta Competent Cells
PCR Amplification for Cloning Gel Verification Protocol
PCR Purification
Qiagen Plasmid DNA Miniprep Kit Protocol
Restriction Enzyme Digestion Protocol Bamhi
Electroporation of E. Coli
Lucigen Pir+ Electroporation of E. coli
Daidzein Pathway Feeding Protocol
Daidzein Feeding and Extraction Protocol
Equol Protocols
Conjugation Protocol
L. casei Conjugation
Landing pad clone screen
Quantification of Equol using HPLC
Housekeeping Protocols
Antibiotics Protocol
Bacterial Glycerol Freezer Stock Protocol
Inoculation + liquid medium Protocol
Lysogeny Broth (LB) Media Protocol
Oligo Suspension + 10µM stock Protocol