Demonstrate engineering success in a part of your project by going through at least one iteration of the engineering design cycle.
We plan to continue this project by redoing our Gibson Assembly so that our gBlock designs are properly placed in their designated plasmid, and that these plasmids are transformed so that they grow in a bacteria colony. From there, we will do in vivo testing to make sure that the reporter protein is translated after E.coli cells come in contact with target miRNA. After we have confirmed the system works via in vivo testing, we will then create a cell-free system using a commercial kit and E.coli cells grown in lab with the desired plasmids containing our devices and reporter proteins, and implement this system within a paper based device.