Here we showcase our contribution to the future IGEM team. We have checked all the original parts related to previous years, and we only found IsmA gene. Here, you can see the data on the ACS gene and the GalU gene on cholesterol absorption, and if future teams do research on cholesterol, they can build on our foundation. We also have some experimental data on oleic acid promoters and cholesterol transporters. I hope to contribute to the future IGEM team.

Cholesterol transport proteins play a crucial role in transferring cholesterol into probiotics. Our experimental data related to cholesterol transport proteins can provide valuable references for future teams. Understanding the functionality and mechanisms of cholesterol transport proteins is essential for researching cholesterol absorption and metabolism, offering valuable insights for future studies.

Oleic acid has the ability to effectively inhibit cholesterol formation, and it is commonly found in various food sources. Oleic acid degrades low-density cholesterol, and it exists abundantly in animal and plant oils in the form of glyceric acid. Moreover, oleic acid has a minimal impact on bacterial growth and can activate the expression of the oleic acid promoter. This information can be a valuable resource for future teams interested in utilizing oleic acid for cholesterol-related applications.

ACS genes play a significant role in anaerobic cholesterol degradation, particularly in the side-chain degradation process. Should future teams undertake cholesterol-related projects, our findings regarding the ACS genes may offer valuable insights and references for their research.

The utilization of the GalU gene can enhance the production of exopolysaccharides (EPS) in E. coli, which, in turn, can increase the adsorption of cholesterol by E. coli Rosetta. EPS also finds applications across various fields in life sciences, making it a versatile resource. Our work lays the foundation for future teams interested in harnessing the GalU gene and its associated EPS production capabilities.

In conclusion, our research has contributed by providing valuable data and insights into cholesterol-related components and processes. We believe that this information will be instrumental in advancing future iGEM projects focusing on cholesterol metabolism and biodegradation. We encourage upcoming iGEM teams to build upon our findings, further expanding the possibilities in this area of research.