In our IGEM journey, we have diligently worked to elevate our contributions, keeping future IGEM teams in mind. Our endeavors encompassed various aspects, from enhancing existing parts to introducing novel approaches and ensuring laboratory safety. In this comprehensive report, we detail our accomplishments and innovations for the benefit of the IGEM community.

We began our journey by enhancing an existing part, connecting the phoA signal peptide gene with the hEGF epidermal growth factor gene. This connection allowed us to label EGF with phoA, facilitating its secretion into the extracellular space. Through meticulous experimentation, including Western Blot and fluorescence microscopy, we confirmed the successful secretion of EGF into the extracellular environment. Our improved design offers valuable insights for future IGEM teams, providing clear instructions for the utilization of signal peptides in the direct delivery of EGF.

Our exploration extended to the adaptability and permeability of bacterial cellulose produced by Squirrel-Beijing-1. We inoculated EcN onto the bacterial cellulose membrane and monitored its survival after one day, mimicking a product usage cycle. Additionally, we conducted experiments to ascertain whether EGF and fatty acids could traverse the bacterial cellulose membrane effectively. These insights offer valuable guidance for future experiments involving bacterial cellulose applications.

Beyond our experiments, we ventured into the realm of innovative skin disease treatment. Acknowledging the limitations of traditional methods, such as antibiotics, hormone medications, and chemical products, we proposed a skin-friendly approach rooted in synthetic biology. Our approach involves engineering bacteria to degrade fatty acids, reduce inflammation, produce growth factors for wound healing, and utilize trace amounts of silver ions to eliminate P. acne. This novel methodology serves as a reference for future teams and holds the potential for broader applications in treating various skin diseases.

In our commitment to safety and responsible laboratory practices, we established a new laboratory safety management system. Our system mandates the completion of laboratory safety questions before any operations commence. Furthermore, we created instructional videos and reminders for laboratory instruments, accessible via QR codes that can be affixed to the equipment for the convenience of subsequent IGEM teams.

In conclusion, our multifaceted contributions aim to enhance the IGEM community's capabilities, promote innovative solutions, prioritize safety, and inspire the next generation of scientists. We remain committed to advancing the field of synthetic biology and look forward to the collaborative efforts of future IGEM teams.