We submitted 12 basic parts and 7 composite parts. Together, they support each other to build the Recircuit system, where different combinations of basic parts form different composite parts. While we didn’t get to test the efficacy of the pathways these parts encode for in vivo, they allow future teams to utilize the modularity of the parts, making their own composite parts. We successfully assembled composite parts BBa_K4728016 to BBa_K4728020 and tested our PhaF phasin mutant (BBa_K4728010) in silico. Through our Project pages, we’ve shown how each part is dependent on other part(s) and all contribute to our engineered PET to PHB pathway.
Other parts we did not work on but would contribute to Recircuit include the many well characterized PETases and MHETases, along with composite parts for PHB synthesis, such as parts by iGEM 2017 Calgary, who helped inspire us this year.
| Part # | Name | Type |
|---|---|---|
| BBa_K4728000 | TPA-inducible promoter | Promoter |
| BBa_K4728001 | TphR (Transcriptional Regulator) | CDS |
| BBa_K4728002 | tphC (Receptor) | CDS |
| BBa_K4728003 | TphA2 (Large subunit of oxygenase component) | CDS |
| BBa_K4728004 | TphA3 (Small subunit of oxygenase component) | CDS |
| BBa_K4728005 | TphB (Dehydrogenase) | CDS |
| BBa_K4728006 | TphA1 (Reductase) | CDS |
| BBa_K4728007 | PhaF | CDS |
| BBa_K4728008 | PhaF mutant | CDS |
| BBa_K4728009 | TpiB (Transmembrane Protein) | CDS |
| BBa_K4728010 | TpiA (Transmembrane Protein) | CDS |
| BBa_K4728011 | TpaK Transporter | CDS |
| BBa_K4728016 | tph operon from Comamonas testosteroni | Composite |
| BBa_K4728017 | TPA-induced promoter - eiraCFP reporter | Composite |
| BBa_K4728018 | Constitutive TphR expression | Composite |
| BBa_K4728019 | TPA transporters expression (TphC, TpiB, TpiA) | Composite |
| BBa_K4728020 | TPA transporter expression (TpaK) | Composite |
| BBa_K4728021 | TPA detection device (using tripartite transporter) | Composite |
| BBa_K4728022 | TPA detection device (with the transporter TpaK) | Composite |
BBa_K4728008, the coding sequence for the mutant PhaF phasin we developed, is an improvement on the wild-type PhaF, submitted as BBa_K4728007.