Results

The transformation of bacterial strains was successful, resulting in cells carrying tachyplesin. However, due to limited time and resources within the scope of the iGEM competition, we were unable to conduct the necessary assessment of tachyplesin's antimicrobial activity against microorganisms colonizing and causing biodeterioration in historic marble monuments, despite this being the primary aim of our project, M.ER.CURE 2.0. This highlights the importance of ongoing research and development in this field to achieve sustainable solutions for the protection of cultural heritage from biodegradation.