In this part collection which ranges from part number BBa_K5017000 to BBa_K5017015, we have successfully submitted fifteen basic parts and one composite part to the Registry. The primary function of this collection lies with the Chimeric Antigen Receptor that we constructed (Part:BBa_K5017000). Parts from BBa_K5017001 to BBa_K5017007 are components of this receptor. Parts from BBa_K5017008 to BBa_K5017015 are designed by Dry Lab with the software DiffAb, using a binding optimization algorithm for the anti-MSLN-scFv (Part: BBa_K5017002). We aim to test these optimized sequences in the future in vitro by comparing the strength of antigen-scFv binding, to see if indeed an optimized sequence has better binding properties.

Part
Part Name
Type
Designed by
Registry link
BBa_K5017000
Chimeric Antigen Receptor (CAR) targeting Mesothelin (MSLN) coupled with GFP-BleoR
DNA
Christos Kalogeropoulos

Nefeli Kosta
BBa_K5017001
CD8a Leader domain
DNA
Christos Kalogeropoulos

Nefeli Kosta
BBa_K5017002
Anti-Mesothelin (anti-MSLN) single chain fragment variable (scFv)
DNA
Christos Kalogeropoulos

Nefeli Kosta
BBa_K5017003
CD8a Hinge domain
DNA
Christos Kalogeropoulos

Nefeli Kosta
BBa_K5017004
NKG2D Transmembrane domain
DNA
Christos Kalogeropoulos

Nefeli Kosta
BBa_K5017005
2B4 domain
DNA
Christos Kalogeropoulos

Nefeli Kosta
BBa_K5017006
IRES Element
DNA
Christos Kalogeropoulos

Nefeli Kosta
BBa_K5017007
GFP-BleoR
DNA
Christos Kalogeropoulos

Nefeli Kosta
BBa_K5017008
Optimized_1_Anti-Mesothelin (anti-MSLN) single chain fragment variable (scFv)
DNA
Vlasios Panagiotis Panagiotou
BBa_K5017009
Optimized_2_Anti-Mesothelin (anti-MSLN) single chain fragment variable (scFv)
DNA
Vlasios Panagiotis Panagiotou
BBa_K5017010
Optimized_3_Anti-Mesothelin (anti-MSLN) single chain fragment variable (scFv)
DNA
Vlasios Panagiotis Panagiotou
BBa_K5017011
Optimized_4_Anti-Mesothelin (anti-MSLN) single chain fragment variable (scFv)
DNA
Vlasios Panagiotis Panagiotou
BBa_K5017012
Optimized_5_Anti-Mesothelin (anti-MSLN) single chain fragment variable (scFv)
DNA
Vlasios Panagiotis Panagiotou
BBa_K5017013
Optimized_6_Anti-Mesothelin (anti-MSLN) single chain fragment variable (scFv)
DNA
Vlasios Panagiotis Panagiotou
BBa_K5017014
Optimized_7_Anti-Mesothelin (anti-MSLN) single chain fragment variable (scFv)
DNA
Vlasios Panagiotis Panagiotou
BBa_K5017015
Optimized_9_Anti-Mesothelin (anti-MSLN) single chain fragment variable (scFv)
DNA
Vlasios Panagiotis Panagiotou