BBa_K4865002

Our aim is to produce a biomaterial that can reduce the complications of skin damage during recovery and support accelerated wound healing. We constructed a composite part, PelB-EGF-R (BBa_K4865002), to achieve this goal. For promoting wound healing, we chose to use the epidermal growth factor, EGF, a single polypeptide which is involved in the regulation of cell proliferation. R is a repetitive region of a spider silk protein gene. And it shows a good mechanical properties and thermal stability, which is a potential medical material (Perry D J. et al, 2010; Tuo Yi, 2019). The N-terminal of this composite part is a secretion signal, pelB, which directs synthesized polypeptides to the E. coli periplasm (Yoon S.H. et al., 2010).