Completed Shark Tank style presentations to the group
Decided to pursue a Type 3 Secretion System Project
Began to set out timeline for future of project with a Gantt chart
04/19/2023
Brainstormed protein disorders to target
Defined Project Version 1: Some diseases cause incorrect protein folding. We will use T3SS to replace these proteins or their function
HP
4/20/2023
Emailed Dr. Ruano-Gallego and Dr. Luis Ángel Fernández from the Spanish Research Council (CSIC) about their success in expressing Type III Secretion Systems in Escherichia Coli K-12
Were instead referred to Dr. Cammie Lesser from Harvard University
Wet Lab
05/11/2023
Meeting with Dr. Lesser from Harvard University
Discussed conceptualization for our project and collaboration, arranged MTA
5/19/2023
Meeting with Dr. Immethun who is a synthetic biologist from Battelle
Received feedback on presentation of our project and the direction/scale of the idea
Researched potential therapeutic proteins/nanobodies to target with delivery system, reached out to authors for more discussion
Began brainstorming protocols
Dry Lab
5/3/2023
Began research on a support vector machine (SVM) model to identify proteins that could secrete through T3SS and successfully refold
Identified DeepT3 as a project of interest for protein identification
5/10/2023
Realized DeepT3 would not work because it was tuned for effector protein identification from Shigella genome
Created list of attributes we could use to fingerprint different proteins of interest
5/17/2023
After meeting with Dr. Lesser, the team pivoted to nanobody research, and scrapped the SVM idea because nanobodies often fold successfully
HP
5/5/2023
Explored potential collaboration with other iGEM teams in Ohio, including Wright State University’s (WSU) team
5/19/2023
Presented the initial Type III Secretion System project to science and technology company, Battelle, to practice our speaking skills and get technical opinions on our project
5/20/2023
Released information about our 2023 project on our Instagram
5/28/2023
Researched other student organizations affiliated with Ohio State to collaborate with them to promote synthetic biology outreach
5/31/2023
Explored options for experiments to present to The Center for Science and Industry (COSI) in the upcoming meeting
Wet Lab
6/15/2023
Asked Dr. Lesser about specific questions and feasibility
6/20/2023
Response from Dr. Lesser which included the sequences for N-terminal secretion sequences
Email included a brief description of Dr.Lesser’s research and gave advice on how to go about the rest of the experiment such as how to start
Dry Lab
6/14/2023
Looked at previous iGem teams to get an idea of what we wanted to do, began work on infographic
6/21/2023
Kickstarter python training with download tutorial, began to think about what professionals to contact
6/28/2023
Found several dry lab professionals to contact, made questionnaire sheet for professionals, python tutorial pandas library
HP
6/2/2023
Zoomed with COSI alongside WSU to plan a date to visit the museum to teach about synthetic biology and present thorough descriptions of our respective 2023 projects
6/7/2023
Met via zoom to discuss experiment ideas for COSI
6/17/2023
Further brainstormed ideas for COSI and general setup
6/21/2023
Met via zoom to discuss additional ways to educate the local community about synthetic biology and type III secretion systems. Explored using 3D models and collaborating with local Girl Scout troops in need of their “Think like an Engineer” journey
Dry Lab
7/5/2023
Went over python assignment to teach how to analyze and graph data in python (libraries, syntax, spacing)
7/12/2023
Biocad discussion & elected team member to research it, reached out to set up meeting with Lindsey (Graduate Student Advisor)
HP
7/2/2023
Posted an extensive description on the contents of the 2023 project on our Instagram
7/11/2023
Met to discuss availability for COSI and the progress made on models and experiments to be presented there
7/21/2023
Finalized experiments to be conducted at COSI and developed procedures for each
Wet Lab
8/8/2023
Conducted lab training videos where Satvik (Graduate Student Wet Lab Advisor) went over how to use each of the devices
8/9/2023
Requested information regarding the plasmid maps for the Flag-tagged Shigella Effector and nanobody that were used as positive controls by Dr. Lesser’s lab
Dry Lab
8/2/2023
Questioned Jay (Wet Lab Lead) for wet lab information, decided to do a modeling approach, planned to meet with a professional Dr. Ramnath for machine learning, added questions to professional questionnaire
8/28/2023
Discussed professionals we have emailed and how they have helped our project
Elijah updated the team on his modeling work
HP
8/3/2023
Extensively detailed remaining tasks and distributed the work equitably
8/14/2023
Collaborated with the Wright State University iGEM team to teach synthetic biology and about our respective projects at the Boonshoft Museum of Discovery
8/15/2023
Posted Instagram stories about our trip to Boonshoft to teach our followers about synthetic biology
8/27/2023
Posted three patient surveys into a broad spectrum of subreddits of patient subgroups affected with gastrointestinal disorders to obtain feedback on willingness to use T3SS therapies
Wet Lab
9/5/2023
The team was informed that the laboratory would not be available until the end of October 2023
Dry Lab
9/13/2023
Started prioritizing the wiki and continuing to work on it
Helped Elijah (Team Co-President) with the modeling
Wiki tutorials/Wiki101
9/28/2023
Continued to work on wiki, got video uploaded, pictures and home page done
Finished wiki writeups needed for Drylab, learning HTML as a team
HP
9/2/2023
Volunteered at COSI with the Wright State University iGEM team to bring synthetic biology to young students
9/3/2023
Included photos from COSI on our Instagram story to show our followers how they can perform easy scientific experiments at their home
9/30/2023
Team attended a science and sustainability event, WestFest, to teach others about synthetic biology and our project
