Parts overview
We designed 64 parts for our project, consisting of 36 basic parts and 28 composite parts, including the vector plasmids used in experiments, DNA sequences successfully mutated through ESM-1v, and primers used for mutagenesis. Our favorite parts are BBa_K4790033 and BBa_K4790061, which encode the most thermally stable PETase we could obtain at present. All parts we used have been submitted to the iGEM Registry according to BioBricks assembly standards. The following are brief tables of our registered parts. For more information about our parts, please visit our Parts Page.
Basic parts
| Name | Type | Description | Designer | Length(bp) |
|---|---|---|---|---|
| BBa_K4790001 | Plasmid | pET-22b(+) | Hongyan Jing | 5817 |
| BBa_K4790025 | Coding | ASR1(node 59)-E125R | Hongyan Jing | 918 |
| BBa_K4790026 | Coding | ASR1(node 59)-P177E | Hongyan Jing | 918 |
| BBa_K4790027 | Coding | ASR1(node 59)-G201A | Hongyan Jing | 918 |
| BBa_K4790028 | Coding | ASR1(node 59)-F213S | Hongyan Jing | 918 |
| BBa_K4790029 | Coding | ASR1(node 59)-I223E | Hongyan Jing | 918 |
| BBa_K4790030 | Coding | ASR1(node 59)-S242G | Hongyan Jing | 918 |
| BBa_K4790031 | Coding | ASR1(node 59)-L246H | Hongyan Jing | 918 |
| BBa_K4790032 | Coding | ASR1(node 59)-K249E | Hongyan Jing | 918 |
| BBa_K4790033 | Coding | ASR1(node 59)-V265T | Hongyan Jing | 918 |
| BBa_K4790034 | Coding | ASR1(node 59)-F275L | Hongyan Jing | 918 |
| BBa_K4790035 | Coding | ASR1(node 59)-C287P | Hongyan Jing | 918 |
| BBa_K4790055 | Coding | ASR1(node 59)-M17A | Yi Zheng | 918 |
| BBa_K4790056 | Coding | ASR1(node 59)-C26A | Yi Zheng | 918 |
| BBa_K4790057 | Coding | ASR1(node 59)-M40E | Yi Zheng | 918 |
| BBa_K4790058 | Coding | ASR1(node 59)-Y46I | Yi Zheng | 918 |
| BBa_K4790059 | Coding | ASR1(node 59)-Q79N | Yi Zheng | 918 |
| BBa_K4790060 | Coding | ASR1(node 59)-N81H | Yi Zheng | 918 |
| BBa_K4790061 | Coding | ASR1(node 59)-W120R | Yi Zheng | 918 |
| BBa_K4790062 | Coding | ASR1(node 59)-L122K | Yi Zheng | 918 |
| BBa_K4790064 | Coding | ASR1(node 59) | Yi Zheng | 918 |
| BBa_K4790067 | Plasmid | PETase-node59 | Yi Zheng | 6405 |
| BBa_K4790068 | Coding | ASR3(node 100) | Yi Zheng | 750 |
| BBa_K4790069 | Coding | ASR4(node 109) | Yi Zheng | 753 |
| BBa_K4790070 | Coding | ASR5(node 111) | Yi Zheng | 750 |
| BBa_K4790071 | Coding | ASR6(node 112) | Yi Zheng | 765 |
| BBa_K4790072 | Coding | ASR7(node 115) | Yi Zheng | 771 |
| BBa_K4790073 | Coding | ASR1(node 59)-Y6A | Yi Zheng | 918 |
| BBa_K4790075 | T7 | T7 promotor | Hongyan Jing | 19 |
| BBa_K4790076 | teminator | T7 terminator | Hongyan Jing | 48 |
| BBa_K4790077 | Tag | 6xHis | Hongyan Jing | 18 |
| BBa_K4790078 | Coding | lac operator | Hongyan Jing | 25 |
| BBa_K4790079 | RBS | RBS | Hongyan Jing | 23 |
| BBa_K4790080 | Signalling | PelB | Hongyan Jing | 66 |
| BBa_K4790081 | Coding | PETase | Hongyan Jing | 792 |
| BBa_K4790108 | Coding | ASR2(node 98) | Hongyan Jing | 1770 |
Composite Parts
| Name | Type | Description | Designer | Length(bp) |
|---|---|---|---|---|
| BBa_K4790074 | Composite | IsPETase/pET-22b(+) | Hongyan Jing | 1035 |
| BBa_K4790082 | Composite | ASR1(node 59)/pET-22b(+) | Hongyan Jing | 1035 |
| BBa_K4790083 | Composite | ASR3(node 100)/pET-22b(+) | Hongyan Jing | 993 |
| BBa_K4790084 | Composite | ASR4(node 109)/pET-22b(+) | Hongyan Jing | 996 |
| BBa_K4790085 | Composite | ASR5(node 111)/pET-22b(+) | Hongyan Jing | 993 |
| BBa_K4790086 | Composite | ASR6(node 112)/pET-22b(+) | Hongyan Jing | 1008 |
| BBa_K4790087 | Composite | ASR7(node 115)/pET-22b(+) | Hongyan Jing | 1014 |
| BBa_K4790088 | Composite | ASR1(node 59)-E125R/pET-22b(+) | Hongyan Jing | 1161 |
| BBa_K4790089 | Composite | ASR1(node 59)-P177E/pET-22b(+) | Hongyan Jing | 1161 |
| BBa_K4790090 | Composite | ASR1(node 59)-G201A/pET-22b(+) | Hongyan Jing | 1161 |
| BBa_K4790091 | Composite | ASR1(node 59)-F213S/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790092 | Composite | ASR1(node 59)-I223E/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790093 | Composite | ASR1(node 59)-S242G/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790094 | Composite | ASR1(node 59)-L246H/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790095 | Composite | ASR1(node 59)-K249E/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790096 | Composite | ASR1(node 59)-V265T/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790097 | Composite | ASR1(node 59)-F275L/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790098 | Composite | ASR1(node 59)-C287P/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790099 | Composite | ASR1(node 59)-M17A/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790100 | Composite | ASR1(node 59)-C26A/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790101 | Composite | ASR1(node 59)-M40E/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790102 | Composite | ASR1(node 59)-Y46I/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790103 | Composite | ASR1(node 59)-Q79N/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790104 | Composite | ASR1(node 59)-N81H/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790105 | Composite | ASR1(node 59)-W120R/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790106 | Composite | ASR1(node 59)-L122K/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790107 | Composite | ASR1(node 59)-Y6A/pET-22b(+) | Yutong Ji | 1161 |
| BBa_K4790109 | Composite | ASR2(node 98)/pET-22b(+) | Hongyan Jing | 2015 |
