New Basic Parts

We registered and uploaded basic parts BBa_K4606001, BBa_K4606002, BBa_K4606003, BBa_K4606004, BBa_K4606005 and BBa_K4606006.
We also uploaded basic parts BBa_K4606007 as a backbone and proved it worked as we expected.

New Composite Parts

We have designed three composite parts (BBa_K4606010, BBa_K4606011 and BBa_K4606012) in order to overexpress the corresponding genes in cells.

Part Number	Part Name	Part Type	Abstract
BBa_K4606001	ZsGreen	Coding	ZsGreen is a fluorescent protein and emits green fluorescence after successful transfection in cells.
BBa_K4606002	Firefly-luciferase	Coding	Firefly-luciferase is a typical enzyme protein consisting of 550 amino acids and can catalyze the oxidation of luciferin to oxyluciferin and then emit bioluminescence.
BBa_K4606003	SMAD4(wild type)	Coding	SMAD4 is a transcription factor that has been reported to be closely associated with osteoarthritis. This 414bp-long fragment of SMAD4 is the head of our recombination device, which has the paired base sequence of miR-3074-5p according to the online repository.
BBa_K4606004	SMAD4(mutant type)	Coding	SMAD4(MUT) was generated by mutating the sequence part of SMAD4(WT) that is paired with miR-3074-5p.
BBa_K4606005	miR-3074-5p coding sequence	Coding	As the coding sequence of miRNA, it can be cleaved to miR-3074-5p after transcription.
BBa_K4606006	miR-3074-5p	RNA	miRNA3074 is over-released in the obese state and is associated with osteoarthritis.
BBa_K4606007	Vector-AmpR	Plasmid_Backbone	Vector-AmpR is a vector plasmid backbone. With this device, we can insert the coding sequence of a novel protein.
BBa_K4606010	Vector-ZsGreen-miR-3074-5p-AmpR	Plasmid	We loaded the genes encoding miR-3074-5p into plasmid vectors. In this way we were able to over-express the miRNA in the target cells.
BBa_K4606011	Vector- Luciferase-SMAD4(WT)-AmpR	Plasmid	We integrated the fragment sequence of the SMAD4(WT) gene in front of the luciferase gene and loaded them together into the plasmid vector. If the SMAD4(WT) sequence is successfully expressed, then the downstream luciferase gene will produce active luciferase.
BBa_K4606012	Vector- Luciferase-SMAD4(MUT)-AmpR	Plasmid	We integrated the fragment sequence of the SMAD4(MUT) gene in front of the luciferase gene and loaded them together into the plasmid vector. If the SMAD4(MUT) sequence is successfully expressed, then the downstream luciferase gene will produce active luciferase.