WILD-TYPE GFP

The BioBrick Part BBa_K1833000 (pT7-eGFP)1 comprises a T7 promoter, RBS, and terminator, with an inserted gene of GFPmut3b. This plasmid was retransformed into E. coli BL21. Upon IPTG induction, bacterial lysates underwent heat tests at varied temperatures for 3 minutes, as indicated in Figure 1. Relative to the untreated control, fluorescence fold change depicted thermal stability trends from 70°C, 80°C, to 90°C, retaining 59%, 12%, and 1% of the GFP signal, respectively. This data provided insights into the innate heat tolerance properties of GFPmut3b protein.

Documentation on the Part's Registry page

We've added new documentation to an existing Part on that Part's Registry page (Part:BBa K1833000)
And the following is the screenshot from the page.