Timeline

Complete record of our project.

12/7/2022

Finding A P.I.

Dr. Martin contacted multiple people from different organizations for the P.I role. Jasmine Beharic, Sabine Waigel, Wolfgang Zacharias

New P.I.

Sabine Waigel from INBRE decided to participate in finding more P.I and funds. She took the offer meaning that our main P.I is Sabine Waigel

1/5/2023
1/23/2023

Contacting Experts

Douglas Lin contacted Robert Bates (A researcher that worked with douglas for his science fair back in 2022 school year).

Meeting

Meeting with Robert Bates, gave tips and information about what we should do for our iGEM team.

Gave further instructions on idea generations + fundings

2/1/2023
2/28/2023

Help From Sabine

Sabine Waigel helps with developing our iGEM Team.

Dr. Martin emails her about the costs of trips, necessary funds, and lab space.

Contacting Experts

Dr. Martin reached out to Dr. Himes asking for more tips, possible funds + lab space.

3/1/2023
3/6/2023

GoFundMe

A GoFundMe Page for Louisville iGEM Team was created.

Contacting Experts

Kalp Poladia attempts to contact Dr. Zhu, however no reply was created.

3/6/2023
4/7/2023

Contacting Experts

Douglas attempts to contact Dr. Yaddanapudi, with no avail.

Contacting Experts

Douglas attempts to contact Dr. Rouchka, with no avail as well.

4/20/2023
5/1/2023

Contacting Experts and Grants

Douglas rettempts emailing Dr. Rouchka and Dr. Yaddanapudi, with no avail.

INBRE Grant was offered, but we never responded as a result of school.

Contacting For Lab Space

Douglas emails Kaya for possible lab space, but no response.

5/22/2023
5/26/2023-5/31/2023

Contacting For Lab Space

More attempts on contacting possible lab space, to no avail as well.

Meeting

Meeting with Sabine Waigel.

6/5/2023
6/9/2023

Project Ideas

Idea on PFAS and PFOA generated.

Idea Approval and New Members

Idea was proposed to Sabine Waigel, which was accepted and became determined. Sabine recommended 3 upcoming freshman in duPont Manual Highschool to be apart of the iGEM Team (Aryan, Vishwaa, and Priyam).

6/11/2023
6/13/2023

More P.I.'s and Funding

Contacted a former duPont Manual High teacher that would be our main P.I>, Mr. Zwanzig.

INBRE has provided 6.5k dollars in fundings

Project Forecast and Registration Complete

Andrea Poole provides detailed explanations of what our project would be like.

INBRE funded the registration fee, meaning Louisville iGEM finally became an official iGEM Team.

6/14/2023
6/15/2023-7/20/2023

Grants and Lab Prerequisites

Attempts to contact grants, all failed.

All members signed lab forms, and got lab training. We obtained lab space.

Project Forecast and Registration Complete

Created a plan to follow for meetings, found a time (Wednesday 5:00pm), and started planning on raising 3000$

8/9/2023
8/16/23

Planning and Research

Insilico research planning with the team.

Fundraising

Team members pass out flyers and spread the word about the project (online, family, school, etc.) to raise money. We raised approximately 2000$ from this.

8/17/23-8/22/23
8/23/23

Meeting

Meeting with Dr. Monsen to talk about reverse screening data bases.

Reverse Screening Proteins

Created Database for reverse screened proteins. Based off of TargetNet, SuperPred, SwissTarget, Etc. We researched to understand correlations of PFAS with other proteins interaction.

8/30/23
9/6/23

Contacting Experts and Fundraising

Contacted Dr. Banrida Wahlang.

Prepared carwash fundings raised about 1000$.

Meeting, Research and Jamboree Fee Paid

Paid jamboree 3000 Dollars.

Meeting with Dr. Banrida Wahlang, she said PPAR ALPHA protein interacts with PFAS.

Started research on PPAR Alpha - stimulation of lipid oxidation - pfas overreacts this system causing there to be oxidative stress.

9/12/23