Parts library.
| Part | Description | Status | Source |
|---|---|---|---|
| BBa_J23119 | Constitutive Family Member | Existing | http://parts.igem.org/Part:BBa_J23119 |
| BBa_J61100 | RBS | Existing | http://parts.igem.org/wiki/index.php/Part:BBa_J61100 |
| BBa_E0040 | Reporter protein (GFP) | Existing | http://parts.igem.org/Part:BBa_E0040 |
| BBa_B0010 | Double Terminator | Existing | http://parts.igem.org/wiki/index.php?title=Part:BBa_B0010 |
| BBa_R0062 | Inducible promoter (Lux pR) | Existing | http://parts.igem.org/Part:BBa_R0062 |
| BBa_E1010 | Reporter protein (RFP) | Existing | http://parts.igem.org/Part:BBa_E1010 |
| BBa_K1493002 | Fusaric acid induced promoter | Existing | http://parts.igem.org/Part:BBa_K1493002 |
| BBa_R0062 | Inducible quorum sensing promoter | Existing | http://parts.igem.org/Part:BBa_R0062 |
| BBa_K4952000 | AHL Synthase (LuxI) from DNA Crystallography Data | New | [1] |
| BBa_K4952001 | Rhizobium leguminosarum signal peptide | New | [2] |
| BBa_K4952002 | LuxR from DNA Crystallography Data | New | [3] |
| BBa_K49520003 | LuxR-G2H | New | [4] [5] |
| BBa_K49520004 | LuxR-G2G | New | [4] [5] |
[1] Chung, Jiwoung et al. “Small-molecule inhibitor binding to an N-acyl-homoserine lactone synthase.” Proceedings of the National Academy of Sciences of the United States of America vol. 108,29 (2011): 12089-94. doi:10.1073/pnas.1103165108
[2] Roest, P. H. “Cloning, Nucleotide Sequencing, and Expression in Escherichia Coli of a Rhizobium Leguminosarum Gene Encoding a Symbiotically Repressed Outer Membrane Protein.” National Center for Biotechnology Information, U.S. National Library of Medicine, 1992, www.ncbi.nlm.nih.gov/nuccore/M69214.
[3] Nguyen, Y et al. “Structural and mechanistic roles of novel chemical ligands on the SdiA quorum-sensing transcription regulator.” mBio vol. 6,2 e02429-14. 31 Mar. 2015, doi:10.1128/mBio.02429-14
[4] Collins, Cynthia H., et al. “Directed evolution of vibrio fischeri luxr for increased sensitivity to a broad spectrum of acyl‐homoserine lactones.” Molecular Microbiology, vol. 55, no. 3, 2004, pp. 712–723, https://doi.org/10.1111/j.1365-2958.2004.04437.x.
[5] Devine, H. J., et al. “V.Fischeri Regulatory Protein (Luxr), Autoinducer (Luxi) Genes, Comple - Nucleotide - NCBI.” National Center for Biotechnology Information, U.S. National Library of Medicine, 1988, www.ncbi.nlm.nih.gov/nuccore/M19039.