The Bye-O-Film project has two primary objectives. First, it aims to develop a sensor for detecting biofilm presence on medical implants. This sensor comprises a biological component, an E. coli biosensor with engineered plasmids for signal detection and communication, and an electronic sensor to relay this information to the patient. The second goal is to implement a phage therapy treatment strategy upon biofilm detection, utilizing Dispersin B to break down the biofilm by degrading polysaccharides. This approach benefits broad applicability, facilitates bacterial conversion to a planktonic form, and increases antibiotic susceptibility, ultimately reducing the required antibiotics for treatment. Additionally, the project utilizes a cyclic-di-GMP-based biosensor to monitor biofilm formation and employs an improved promotor (originally designed by the CUG China 2022 iGEM team) to control gene transcription. This page includes an overview of the biological parts made and designed in our project. Each part contains a hyperlink to the iGEM repository. To learn more about the genetic design approach, visit the Engineering page. To see the final results, head over to the Results page.

Figure 1: An overview of the created constructs during the Bye-o-film project, color coded based on the function of the construct (left) with a more detailed description of the particular constructs (right). Please visit the Engineering page for a comprehensive overview of the design strategies for the parts created. Image created using the resources from Slidesgo and Freepik.