Parts

Bioparts are the basic building blocks of the iGEM project. Here, we constructed a catalytic lignan glycoside (BBa_K4824000) biopart (Figure 1). Based on the reported transcriptomic data of I. indigotica, the sequences of genes with glycosyltransferase functions were screened and a phylogenetic tree analysis was conducted with the functionally characterized lignan UGTs, and UGT72B2 was selected as the candidate, which the main component of the biopart. We then constructed expression vectors using homologous recombination and ultimately verified protein function.